Dataset Information

TMED10 INHIBITION SUPPRESSES CELL-SURFACE PD-1 EXPRESSION AND REVERSES T CELL DYSFUNCTION

ABSTRACT: PD-1 immune checkpoint blockade (ICB) is revolutionizing cancer therapy, but little is known about the mechanisms governing its expression. In a whole-genome, dual-marker FACS-based CRISPR/Cas9 screen in primary murine CD8 T cells, we found that inactivation of the EMP24/GP25L/p24 protein family, most prominently TMED10, reduced PD-1 cell-surface stability.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): T Cell, T-lymphocyte

DISEASE(S): Melanoma

SUBMITTER: Onno Bleijerveld

LAB HEAD: Maarten Altelaar

PROVIDER: PXD026984 | Pride | 2025-05-06

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20190513_HFX_p329_Proteome_Tmed10KO_01.raw	Raw
	20190513_HFX_p329_Proteome_Tmed10KO_02.raw	Raw
	20190513_HFX_p329_Proteome_Tmed10KO_03.raw	Raw
	20190513_HFX_p329_Proteome_Tmed10KO_04.raw	Raw
	20190513_HFX_p329_Proteome_Tmed10KO_05.raw	Raw

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Publications

TMED inhibition suppresses cell surface PD-1 expression and overcomes T cell dysfunction.

Vredevoogd David W DW Apriamashvili Georgi G Levy Pierre L PL Sinha Sanju S Huinen Zowi R ZR Visser Nils L NL de Bruijn Beaunelle B Boshuizen Julia J van Hal-van Veen Susan E SE Ligtenberg Maarten A MA Bleijerveld Onno B OB Lin Chun-Pu CP Díaz-Gómez Judit J Sánchez Santiago Duro SD Markovits Ettai E Simon Nieto Juan J van Vliet Alex A Krijgsman Oscar O Markel Gal G Besser Michal J MJ Altelaar Maarten M Ruppin Eytan E Peeper Daniel S DS

Journal for immunotherapy of cancer 20241107 11

<h4>Background</h4>Blockade of the programmed cell death protein 1 (PD-1) immune checkpoint (ICB) is revolutionizing cancer therapy, but little is known about the mechanisms governing its expression on CD8 T cells. Because PD-1 is induced during activation of T cells, we set out to uncover regulators whose inhibition suppresses PD-1 abundance without adversely impacting on T cell activation.<h4>Methods</h4>To identify PD-1 regulators in an unbiased fashion, we performed a whole-genome, fluoresce ...[more]

PMID: 39510795

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