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RNA-mediated nucleosome depletion is required for elimination of transposon-derived DNA

ABSTRACT: To study the role of chromatin remodeler during programmed genome reorganization in Paramecium tetraurelia. Paramecium ISWI1 was tagged with 3 FLAG and HA at its C-terminal. The recombinant plasmid was transformed into Paramecium and used for co-immunoprecipitation and Mass spectrometry studies to identify novel interacting partners of Paramecium ISWI1 protein that modulates the elimination of transposable elements.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Paramecium Tetraurelia

TISSUE(S): Eukaryotic Cell, Cell Culture

SUBMITTER: Manfred Heller

LAB HEAD: Mariusz Nowacki

PROVIDER: PXD027206 | Pride | 2023-03-11

REPOSITORIES: Pride

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Chromatin remodeling is required for sRNA-guided DNA elimination in Paramecium.

Singh Aditi A Maurer-Alcalá Xyrus X XX Solberg Therese T Häußermann Lilia L Gisler Silvan S Ignarski Michael M Swart Estienne C EC Nowacki Mariusz M

The EMBO journal 20221011 22

Small RNAs mediate the silencing of transposable elements and other genomic loci, increasing nucleosome density and preventing undesirable gene expression. The unicellular ciliate Paramecium is a model to study dynamic genome organization in eukaryotic cells, given its unique feature of nuclear dimorphism. Here, the formation of the somatic macronucleus during sexual reproduction requires eliminating thousands of transposon remnants (IESs) and transposable elements scattered throughout the germl ...[more]

PMID: 36221862

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Identification of the Polycomb repressive complex 2 and its interacting proteins in Paramecium tetraurelia

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Structural maintenance of chromosomes (SMCs) proteins are required for developmental DNA elimination in Paramecium tetraurelia

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Control of developmentally primed erythroid genes by combinatorial corepressor actions

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2023-02-01 | PXD033348 | Pride

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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