Proteomics

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Proteomic Profile of Mesenchymal Stromal Cell and Extracellular Vesicle in Normoxic and Hypoxic Conditions

ABSTRACT: The physiological niche of bone marrow is hypoxic. However, the artificial growth of bone marrow derived-mesenchymal stromal cells (MSC)s very often occurs in normoxia which may impact the protein expression with immunomodulatory properties. Thus, cell viability and respiration were assessed as well as the proteomic profile of MSCs and their EVs submitted to normoxia (21% O2) and hypoxia (1% O2) for 48 hours were evaluated. MSCs and EVs total protein were isolated for mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Cell Culture, Mesenchymal Stem Cell Of The Bone Marrow

DISEASE(S): Disease Free

SUBMITTER: Pedro Silva  

LAB HEAD: Pedro Leme Silva

PROVIDER: PXD027718 | Pride | 2026-02-09

REPOSITORIES: Pride

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20200617_02_Amostra16_01.sqt Other
20200617_02_Amostra16_01__1_.raw Raw
20200617_02_Amostra16_02.raw Raw
20200617_02_Amostra16_02.sqt Other
20200617_02_Amostra16_03.raw Raw
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Publications

<h4>Background</h4>During pulmonary arterial hypertension (PAH), cardiac cells develop a hypertrophic and apoptosis-resistant phenotype. Mesenchymal stromal cell (MSC) therapy has been shown to mitigate pulmonary vascular remodeling in PAH; however, successful application is limited by low potency and the need for a high number of MSCs. MSCs exposed to hypoxia release more extracellular vesicles (EV)s with different content than normoxia. We aimed to evaluate the proteomic profile and therapeuti  ...[more]

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