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Proteomic characterization of sorted peroxisomes

ABSTRACT: Proteomic analysis of sorted peroxysomes (old, young, and middle-aged).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Tommaso De Marchi

LAB HEAD: Pekka Katajisto

PROVIDER: PXD028679 | Pride | 2025-03-04

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	HS_sp_CANnISO_20200224.fasta	Fasta
	TDM_M2011_201.raw	Raw
	TDM_M2011_202.raw	Raw
	TDM_M2011_203.raw	Raw
	TDM_M2011_205.raw	Raw

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Publications

Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division.

Bui Hien H Andersson Simon S Sola-Carvajal Agustin A De Marchi Tommaso T Vähäkangas Eliisa E Holopainen Minna M House Andrew H AH Rovenko Bohdana M BM Englund Johanna I JI Kasper Maria M Kuuluvainen Emilia E Käkelä Reijo R Hietakangas Ville V Niméus Emma E Katajisto Pekka P

Nature communications 20250426 1

Selective inheritance of sub-cellular components has emerged as a mechanism guiding stem cell fate after asymmetric cell divisions. Peroxisomes play a crucial role in multiple metabolic processes such as fatty acid metabolism and reactive oxygen species detoxification, but the apportioning of peroxisomes during stem cell division remains understudied. Here, we develop a mouse model and labeling technique to follow the dynamics of distinct peroxisome age-classes, and find that old peroxisomes are ...[more]

PMID: 40287409

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