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IP-MS/MS analysis of UV induced RPII215 interactome

ABSTRACT: Drosophila Melanogaster has been extensively used as a model system to study ionizing radiation and chemical induced mutagenesis, double strand break repair and recombination. However, there are only limited studies on nucleotide excision repair in this important model organism. In this study, we immunopreciptated DNA-directed RNA polymerase II (RPII215) complex from untreated and UV iradiated drosophila S2 cells and identified the protein that interact with it by mass spectrometry.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Li Wang

LAB HEAD: Aziz Sancar

PROVIDER: PXD028924 | Pride | 2022-04-04

REPOSITORIES: Pride

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CSB-independent, XPC-dependent transcription-coupled repair in Drosophila.

Deger Nazli N Cao Xuemei X Selby Christopher P CP Gulec Saygin S Kawara Hiroaki H Dewey Evan B EB Wang Li L Yang Yanyan Y Archibald Sierra S Selcuk Berkay B Adebali Ogun O Sekelsky Jeff J Sancar Aziz A Liu Zhenxing Z

Proceedings of the National Academy of Sciences of the United States of America 20220301 9

Drosophila melanogaster has been extensively used as a model system to study ionizing radiation and chemical-induced mutagenesis, double-strand break repair, and recombination. However, there are only limited studies on nucleotide excision repair in this important model organism. An early study reported that Drosophila lacks the transcription-coupled repair (TCR) form of nucleotide excision repair. This conclusion was seemingly supported by the Drosophila genome sequencing p ...[more]

PMID: 35217627

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