Proteomic analysis of peripheral blood mononuclear cells isolated from patients with pulmonary tuberculosis: a pilot study from Zanzibar, Tanzania

ABSTRACT: This study aimed at exploring the proteomic profile of PBMCs to predict response to treatment in pulmonary tuberculosis (PTB). This was a pilot study conducted among 8 adult patients from Zanzibar, Tanzania with confirmed PTB. Blood samples were collected at baseline, at 2 months of treatment, and at the end of 6 months of treatment. Proteins were extracted from PBMCs and analysed using LC‑MS/MS‑based label‑free quantitative proteomics. Overall, 3,530 proteins were quantified across the samples, and 12 differentially expressed proteins were identified at both 2 months of treatment and at treatment completion, which were involved in cellular and metabolic processes, as well as binding and catalytic activity. Seven were downregulated proteins (HSPA1B/HSPA1A, HSPH1, HSP90AA1, lipopolysaccharide binding protein, complement component 9, calcyclin-binding protein, and protein transport protein Sec31A), and 5 proteins were upregulated (SEC14 domain and spectrin repeat-containing protein 1, leucine-rich repeat-containing 8 VRAC subunit D, homogentisate 1,2-dioxygenase, NEDD8-activating enzyme E1 regulatory subunit, and N-acetylserotonin O-methyltransferase-like protein). The results showed that proteome analysis of PBMCs can be used as a novel technique to identify potential biomarkers to assess treatment efficacy in PTB. The novel proteins elucidated in this work may provide new insights for understanding PTB pathogenesis, treatment, and prognosis.  

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood

DISEASE(S): Tuberculosis

SUBMITTER: Even Birkeland  

LAB HEAD: Tehmina Mustafa

PROVIDER: PXD029634 | Pride | 2023-03-11

REPOSITORIES: Pride