Dataset Information

Deep proteomic analysis of human hippocampus micro-sections provides a new avenue to identify hippocampal subfield heterogeneity in the protein expression

ABSTRACT: Hippocampus has drawn an immense attention from researchers and clinicians worldwide due to its clinical importance in various neurodegenerative disorders. Each hippocampal subfield is known to perform distinct functions and are selectively vulnerable to environmental, physiological, and clinical factors. Therefore, proteomic analysis of hippocampus at subfield level is increasingly important, however, an in-depth proteomic analysis of human hippocampus at its subfield level is not well established to date. In this study, we compared the sub-proteome of the hippocampus- the cornu ammonis sectors (CA1, CA2, CA3 and CA4), and dentate gyrus (DG) from healthy adult human cohorts using high resolution mass spectrometry. We procured hippocampal subfields from the archived FFPE tissue sections by performing manual micro-dissection and conducted TMT label quantification-based proteomic analysis that resulted in the identification of 5,593 proteins, of which 890 proteins were differentially abundant at ≥1.5-fold cut-off across all subfields. Gene Ontology enrichment analysis was performed to uncover the biological processes, pathways and cellular localization associated with each subfield of hippocampus. IHC-based validation of selected proteins were also carried out in an independent set of hippocampus from normal subjects. We believe that our findings will effectively pave a way for further analysis of the hippocampal subdivisions and understand the etiopathology of many neurological disorders in future.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Keshava Prasad T. S.

LAB HEAD: Dr. T. S. Keshava Prasad

PROVIDER: PXD029697 | Pride | 2022-10-14

REPOSITORIES: Pride

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Publications

Proteomic Analysis of Adult Human Hippocampal Subfields Demonstrates Regional Heterogeneity in the Protein Expression.

Mol Praseeda P Gopalakrishnan Lathika L Chatterjee Oishi O Mangalaparthi Kiran K KK Kumar Manish M Durgad Shwetha S SS Nair Bipin B Shankar Susarla K SK Mahadevan Anita A Prasad Thottethodi Subrahmanya Keshava TSK

Journal of proteome research 20220830 10

<b>Background:</b> Distinct hippocampal subfields are known to get affected during aging, psychiatric disorders, and various neurological and neurodegenerative conditions. To understand the biological processes associated with each subfield, it is important to understand its heterogeneity at the molecular level. To address this lacuna, we investigated the proteomic analysis of hippocampal subfields─the cornu ammonis sectors (CA1, CA2, CA3, CA4) and dentate gyrus (DG) from healthy adult human coh ...[more]

PMID: 36039803

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Dataset Information

Deep proteomic analysis of human hippocampus micro-sections provides a new avenue to identify hippocampal subfield heterogeneity in the protein expression

Publications

Proteomic Analysis of Adult Human Hippocampal Subfields Demonstrates Regional Heterogeneity in the Protein Expression.

Similar Datasets

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