Dataset Information

S-nitrosation of Arabidopsis thaliana Protein Tyrosine Phosphatase 1 (AtPTP1) prevents its irreversible oxidation by hydrogen peroxide

ABSTRACT: Tyrosine-specific protein tyrosine phosphatases (Tyr-specific PTPases) are key signalling enzymes catalyzing the removal of the phosphate group from phosphorylated tyrosine residues on target proteins. This post-translational modification notably allows the regulation of mitogen-activated protein kinase (MAPK) cascades during defense reactions. Arabidopsis thaliana protein tyrosine phosphatase 1 (AtPTP1), the only Tyr-specific PTPase present in this plant, acts as a repressor of H2O2 production and regulates the activity of MPK3/MPK6 MAPKs by direct dephosphorylation. Here, we report that recombinant His-AtPTP1 protein activity is directly inhibited by H2O2 and nitric oxide (NO) exogenous treatments. The effects of NO are exerted by S-nitrosation, i.e. the formation of a covalent bond between NO and a reduced cysteine residue. This post-translational modification targets the catalytic cysteine C265 and could protect the AtPTP1 protein from its irreversible oxidation by H2O2. This mechanism of protection could be a conserved mechanism in all plant PTPases

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Carole Pichereaux

LAB HEAD: Besson-Bard Angelique

PROVIDER: PXD030071 | Pride | 2022-04-04

REPOSITORIES: Pride

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Publications

S-Nitrosation of Arabidopsis thaliana Protein Tyrosine Phosphatase 1 Prevents Its Irreversible Oxidation by Hydrogen Peroxide.

Nicolas-Francès Valérie V Rossi Jordan J Rosnoblet Claire C Pichereaux Carole C Hichami Siham S Astier Jeremy J Klinguer Agnès A Wendehenne David D Besson-Bard Angélique A

Frontiers in plant science 20220211

Tyrosine-specific protein tyrosine phosphatases (Tyr-specific PTPases) are key signaling enzymes catalyzing the removal of the phosphate group from phosphorylated tyrosine residues on target proteins. This post-translational modification notably allows the regulation of mitogen-activated protein kinase (MAPK) cascades during defense reactions. Arabidopsis thaliana protein tyrosine phosphatase 1 (AtPTP1), the only Tyr-specific PTPase present in this plant, acts as a repressor of H<s ...[more]

PMID: 35222471

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Dataset Information

S-nitrosation of Arabidopsis thaliana Protein Tyrosine Phosphatase 1 (AtPTP1) prevents its irreversible oxidation by hydrogen peroxide

Publications

S-Nitrosation of <i>Arabidopsis thaliana</i> Protein Tyrosine Phosphatase 1 Prevents Its Irreversible Oxidation by Hydrogen Peroxide.

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