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TMT-switch assay for the detection of SNO

ABSTRACT: To maximize SNO-proteome coverage and ensure capture of all modified sites, different experimental configurations using both iodo- and cysTMT were evaluated.

INSTRUMENT(S): LTQ Orbitrap Velos, LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Heaseung Chung

LAB HEAD: Heaseung Sophia Chung

PROVIDER: PXD000741 | Pride | 2016-12-23

REPOSITORIES: Pride

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Dual Labeling Biotin Switch Assay to Reduce Bias Derived From Different Cysteine Subpopulations: A Method to Maximize S-Nitrosylation Detection.

Chung Heaseung Sophia HS Murray Christopher I CI Venkatraman Vidya V Crowgey Erin L EL Rainer Peter P PP Cole Robert N RN Bomgarden Ryan D RD Rogers John C JC Balkan Wayne W Hare Joshua M JM Kass David A DA Van Eyk Jennifer E JE

Circulation research 20150903 10

<h4>Rationale</h4>S-nitrosylation (SNO), an oxidative post-translational modification of cysteine residues, responds to changes in the cardiac redox-environment. Classic biotin-switch assay and its derivatives are the most common methods used for detecting SNO. In this approach, the labile SNO group is selectively replaced with a single stable tag. To date, a variety of thiol-reactive tags have been introduced. However, these methods have not produced a consistent data set, which suggests an inc ...[more]

PMID: 26338901

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