Dataset Information

Tracking prostate carcinogenesis over time through urine proteome profiling in an animal model: an exploratory approach

ABSTRACT: The urgent need for new tools for the early diagnosis of prostate cancer (PCa) has prompted us to investigate the proteomic profile of urine during the course of prostate carcinogenesis. Using an established animal model of prostate adenocarcinoma, we performed a comprehensive analysis of the proteomic profile of urine from healthy animals and animals with adenocarcinoma at two time points. At the second time point of prostate carcinogenesis, the incidence of pre-neoplastic and neoplastic lesions was 100%. GeLC-MS/MS and subsequent bioinformatic analysis revealed several proteins involved in prostate carcinogenesis. Increased levels of retinol-binding protein 4 and decreased levels of cadherin-2 appear to be characteristic of early stages of the disease, whereas increased levels of enolase-1 and T-kininogen 2 and decreased levels of isocitrate dehydrogenase 2 describe more advanced stages. With increasing age, urinary levels of clusterin and corticosteroid-binding globulin increase and neprilysin levels decrease, all of which appear to play a role in prostatic hyperplasia or carcinogenesis. The present exploratory analysis could be considered an important starting point for studies targeting specific human urinary proteins for the early detection of age-related maladaptive changes in the prostate that may lead to cancer.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Urine

DISEASE(S): Prostate Cancer

SUBMITTER: Rui Vitorino

LAB HEAD: Rui Vitorino

PROVIDER: PXD034432 | Pride | 2022-08-12

REPOSITORIES: Pride

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Publications

Tracking Prostate Carcinogenesis over Time through Urine Proteome Profiling in an Animal Model: An Exploratory Approach.

Moreira-Pais Alexandra A Nogueira-Ferreira Rita R Reis Stephanie S Aveiro Susana S Barros António A Melo Tânia T Matos Bárbara B Duarte José Alberto JA Seixas Fernanda F Domingues Pedro P Amado Francisco F Fardilha Margarida M Oliveira Paula A PA Ferreira Rita R Ferreira Rita R Vitorino Rui R

International journal of molecular sciences 20220708 14

Prostate cancer (PCa) is one of the most lethal diseases in men, which justifies the search for new diagnostic tools. The aim of the present study was to gain new insights into the progression of prostate carcinogenesis by analyzing the urine proteome. To this end, urine from healthy animals and animals with prostate adenocarcinoma was analyzed at two time points: 27 and 54 weeks. After 54 weeks, the incidence of pre-neoplastic and neoplastic lesions in the PCa animals was 100%. GeLC-MS/MS and s ...[more]

PMID: 35886909

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Project description:Background: Sepsis-associated encephalopathy (SAE) is a common and severe complication of sepsis. While several studies have reported the proteomic alteration in plasma, urine, heart, etc. of sepsis, few research focused on the brain tissue. This study aims at discovering the differentially abundant proteins in the brains of septic rats to identify biomarkers of SAE. Methods: The Prague-Dawley rats were randomly divided into sepsis (n = 6) or sham (n = 6) groups, and then the whole brain tissue was dissected at 24 h after surgery for further protein identification by TMT-LC–MS/MS-based proteomics. Ingenuity pathway analysis, Gene ontology knowledgebase, and STRING database are used to explore the biological significance of proteins with altered concentration. Results: Among the total of 3163 proteins identified in the brain tissue, 57 were increased while 38 were decreased in the sepsis group compared to the sham group. Bioinformatic analyses suggest that the differentially abundant proteins are highly related to cellular microtubule metabolism, energy production, nucleic acid metabolism, neurological disease, etc. Additionally, acute phase response signaling was possibly activated and PI3K/AKT signaling was suppressed during sepsis. An interaction network established by IPA revealed that Akt1, Gc-globulin, and ApoA1 were the core proteins. The increase of Gc-globulin and the decrease of Akt1 were confirmed by Western blot. Conclusions: Based on the multifunction of these proteins in several brain diseases, we first propose that Gc-globulin, ApoA1, PI3K/AKT pathway, and acute phase response proteins (hemopexin and cluster of alpha-2-macroglobulin) can potentially be diagnosis biomarkers, therapeutic targets, and prognosis indicators of SAE. These results may provide new insights into the pathologic mechanism of SAE, yet further research is required to explore the functional implications and clinical applications of the differentially abundant proteins in the brains of sepsis group.

Dataset Information

Tracking prostate carcinogenesis over time through urine proteome profiling in an animal model: an exploratory approach

Publications

Tracking Prostate Carcinogenesis over Time through Urine Proteome Profiling in an Animal Model: An Exploratory Approach.

Similar Datasets

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