Human HEK293 Gemin5 phosphorylation site T897 mutants LC-MSMS

ABSTRACT: Identification of cellular proteins interacting with Gemin5 phosphorylation mutants in T897 residue. We used the T897A mutant as phosphorylation defective protein and the T897E mutant as phosphomimetic. P85 C-terminal fragment of Gemin5 has been used to purify the protein complexes associated by Tandem Affinity Purification. The study has been performed using two biological replicates of each bait protein: p85-T897A and p85-T897E.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Rosario Francisco-Velilla  

LAB HEAD: Encarnacion Martinez-Salas

PROVIDER: PXD035227 | Pride | 2023-03-11

REPOSITORIES: Pride