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LC-MSMS analysis of bear muscle mitochondria during hibernation

ABSTRACT: During hibernation, the physiology of bears is characterized by strong metabolic depression. In skeletal muscles, lipids are used as the main energy fuels although their oxidation rate is reduced, and glycolysis appears maintained likely feeding the Cori cycle. Mitochondria no doubt play an essential role in such an adaptation. However, neither their activity nor their protein content had been analysed earlier. To fill this gap, this study analysed the bear (Ursus arctos) skeletal muscle mitochondrial fraction using shotgun proteomics to compare hibernation to the summer-active period.

INSTRUMENT(S):

ORGANISM(S): Ursus Arctos Arctos (european Brown Bear)

TISSUE(S): Skeletal Muscle

SUBMITTER: Fabrice BERTILE

LAB HEAD: Sarah Cianférani

PROVIDER: PXD036916 | Pride | 2026-03-02

REPOSITORIES: Pride

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			Action	DRS
	MQ_Results.zip	Other
	QE30684CB.raw	Raw
	QE30685CB.raw	Raw
	QE30688CB.raw	Raw
	QE30689CB.raw	Raw

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Publications

The Preservation of Muscle Mitochondrial Machinery During Hypometabolic Hibernation in Scandinavian Brown Bears (Ursus arctos).

Bergouignan Audrey A Noone John J Brun Charlotte C Cussonneau Laura L Geffroy Alexandre A Coudy-Gandilhon Cecile C Chery Isabelle I Evans Alina Lynn AL Arnemo Jon Martin JM Kindberg Jonas J Gauquelin-Koch Guillemette G O'Gorman Donal D Lefai Etienne E Bertile Fabrice F

Acta physiologica (Oxford, England) 20260401 4

<h4>Aim</h4>Unlike humans, brown bears (Ursus arctos) uniquely preserve skeletal muscle mass and function during months of hibernation despite prolonged fasting and inactivity. We investigated how mitochondrial energetics respond in skeletal muscle to support this remarkable resilience.<h4>Methods</h4>Muscle biopsies from eight wild brown bears were collected during hibernation and again in the active summer season. We assessed mitochondrial respiration using high-resolution respirometry and eva ...[more]

PMID: 41725390

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Quantitative proteomics of brown bear muscle during hibernation

Project description:During hibernation, the physiology of bears is characterized by strong metabolic depression. In skeletal muscles, lipids are used as the main energy fuels although their oxidation rate is reduced, and glycolysis appears maintained likely feeding the Cori cycle. For a better knowledge on proteome remodelling during hibernation, this study analysed the bear (Ursus arctos) skeletal muscle proteome using shotgun proteomics to compare hibernation to the summer-active period.

2026-03-02 | PXD060503 | Pride

Biochemical Foundations of Health and Energy Conservation in Hibernating Free-Ranging Subadult Brown Bear Ursus arctos

Project description:Brown bears (Ursus arctos) hibernate for 5-7 months without eating, drinking, urinating and defecating at a metabolic rate of only 25% of the summer activity rate. Nonetheless, they emerge healthy and alert in spring. We analyzed the physiological adaptations for hibernation by comparing the proteome, metabolome, and hematologic features of blood from hibernating and active free-ranging sub-adult brown bears with a focus on conservation of health and energy. We found that the concentrations of most plasma proteins and white blood cell types decreased during hibernation. Strikingly, antimicrobial defense proteins increased in concentration. Central functions in hibernation involving the coagulation response and protease inhibition, as well as lipid transport and metabolism, were upheld by increased levels of very few key or broad-specificity proteins. A dramatic 45-fold increase in sex-hormone-binding-globulin SHBG levels during hibernation draws, for the first time, attention to its significant but unknown role in maintaining hibernation physiology. We propose that energy for the costly protein synthesis is reduced by three mechanisms, (i) dehydration, which increases protein concentration without de novo synthesis; (ii) reduced protein degradation rates due to a 6°C reduction in body temperature, decreased protease activity, and increased plasma viscosity; and (iii) a marked redistribution of energy resources only increasing de novo synthesis of few key proteins. This comprehensive global data identified novel biochemical strategies for bear adaptations to the extreme condition of hibernation, and have implications for our understanding of physiology in general.

2016-09-27 | PXD003946 | Pride

Plasma proteomics data of hibernating and active wild Scandinavian brown bears

Project description:Here we provide mass-spectrometry based plasma proteomics data of hibernating and active wild Scandinavian brown bears (Ursus arctos arctos). The brown bear hibernates for half the year. Despite obesity and the prolonged period of inactivity, bears show no signs of the harmful effects associated with these conditions in humans. Thus, the hibernating bear is a protentional translational model for addressing these complications in humans. We analyzed plasma samples from 14 subadult 2- to 3-year-old (y/o) bears (6 males and 8 females) collected both during hibernation and active state, and for some for the bears during two seasons, resulting in a total of 38 analyzed plasma samples. In triplicates, the proteins in the plasma samples were unfolded and reduced. To increase depth of the analysis and the chance to detect low molecular weight proteins and peptides, we filtered samples with a 50K MWCO filter with the aim to deplete larger proteins. The proteins in the permeate were then tryptically digested, desalted, and analyzed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Protein identification and quantification was performed with the MaxQuant software searching against an Ursus arctos horribilis protein database.

2021-12-21 | PXD030482 | Pride

Resting skeletal muscle reduces ATP turnover during hibernation

Project description:In mammals, loss of food intake and reduced mechanical loading/activity of skeletal muscles leads to a very rapid loss in mass and function. However, during hibernation in bears, despite spending months without feeding and with very modest muscle activity, only moderate muscle wasting is observed. Part of this tissue sparing is due to a highly reduced metabolic activity in almost all tissues, including skeletal muscle. Interestingly, myosin, one of the most abundant proteins in skeletal muscle, can have different metabolic activities in inactive muscle. Therefore, to evaluate the functional and metabolic alterations in hibernating muscles, we performed an analysis on a single muscle fiber level. Individual fibers were taken from biopsies of the same bears either during hibernation or during the active phase in the summer. We confirm that muscle fibers from hibernating bears show no loss of fiber size and a mild reduction in force generating capacity. However, ATPase activity of single muscle fibers taken from hibernating bears show a significant reduction in ATPase activity, which is due to a reduced ATP turnover by myosin. By performing a single fiber proteomics analysis, we could determine in a fiber type specific manner that muscle fibers undergo a major remodeling of their proteome. Both type 2A and type 1/2A mixed fibers show a marked reduction in mitochondrial proteins during hibernation, with a decrease in proteins linked to the TCA cycle and mitochondrial translation.

2024-12-06 | PXD050980 | Pride

miRNA profiling by Illumina sequencing technology

Project description:miRNAs are 19-25 nucleotides long small RNAs now well-known for their regulatory roles in the development and diseases through post-transcriptional and translational controls in a wide range of species. Mammalian hibernation is a physiological process involving dramatic metabolic suppression and cellular reorganization, during which miRNAs may play an important role. We systematically analyzed the miRNAs in the liver of an extreme hibernating species, arctic ground squirrels (Spermophilus parryii), during two stages of hibernation compared to non-hibernating animals by massively parallel Illumina sequencing technology. We identified more than 200 ground squirrel miRNAs including novel miRNAs specific to ground squirrel and a fast-evolving miRNA cluster that also showed significant differential expression during hibernation. Integrating with Agilent miRNA microarray and Real-time PCR results, we identified that mir-211, mir-378, mir-184, mir-200a, and mir-320 were significantly under-expressed during hibernation, whereas mir-144, mir-486, mir-451, mir-142-5p, and mir-1 were over-expressed. Analyses of the their target genes suggested that these miRNAs could play an important role to suppress tumor progression and cell growth during hibernation. Investigation of microRNA changes in arctic ground squirrel livers during Early Arousal(EA), Late Topor(LT), and Post-Reproduction(PR) stages.

2010-11-30 | E-GEOD-19807 | biostudies-arrayexpress

Brown bear skeletal muscle 2D-DIGE-LC-MSMS

Project description:Muscle atrophy is one of the main deleterious consequences of ageing and physical inactivity. Although basic knowledge regarding the underlying mechanisms of muscle atrophy is continuously growing, there are still no efficient therapeutic strategies for its prevention and treatment. Hibernating bears exhibit a strong and unique ability to preserve muscle mass in conditions where muscle atrophy is observed in humans. However, underlying mechanisms have not been understood yet. To fill this gap, the aim of this study was to characterize changes in the bear muscle proteome during hibernation versus the active period. Muscle biopsies were obtained from Ursus arctos bears.

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LC-MSMS analysis of HFF1 cells exposed to bear and bovine serum

Project description:The serum of hibernating bears has been shown to exhibit cytoprotective, anticatabolic effects when applied to human or mouse skeletal muscle cells. It is also able to reduce mitochondrial respiration in cells obtained from bear adipose tissue. Its action notably might involve reprogramming of transcriptional profiles, as shown in brown bear adipocytes and more recently in human muscle cells. However, the effects of bear serum on human cells has not been evaluated yet at the proteome level. The main objective of this study was to fill this gap by comparing the effects of hibernating bear serum with those of non-hibernating bear serum and foetal bovine serum on human fibroblasts.

2025-12-08 | PXD069199 | Pride

An evolutionary conserved, immobility associated antithrombotic mechanism

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