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Proteomics and Metabolomics based analysis of Metabolic changes in a swine model of Pulmonary Hypertension

ABSTRACT: Pulmonary vein stenosis (PVS) is a rare type of Pulmonary Hypertension (PH), which impacts the flow and pressure within the lung tissue and vasculature resulting in endothelial dysfunction and metabolic changes. We used a pig model in order to mimic PH post PVS using pulmonary vein banding (PVB) of the lower lobes for 12 weeks to investigate the alteration of pressure and flow which provides an impetus for development of PH. In order to develop site-specific therapies, our current study aimed to employ proteomics and metabolomics on both the upper and lower lobes of the pig lung to identify compartment-specific metabolic alterations.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Lung

DISEASE(S): Pulmonary Hypertension

SUBMITTER: Bachuki Shashikadze

LAB HEAD: Thomas Fröhlich

PROVIDER: PXD038982 | Pride | 2023-04-20

REPOSITORIES: Pride

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Proteomics- and Metabolomics-Based Analysis of Metabolic Changes in a Swine Model of Pulmonary Hypertension.

Sen Payel P Shashikadze Bachuki B Flenkenthaler Florian F Van de Kamp Esther E Tian Siyu S Meng Chen C Gigl Michael M Fröhlich Thomas T Merkus Daphne D

International journal of molecular sciences 20230302 5

Pulmonary vein stenosis (PVS) causes a rare type of pulmonary hypertension (PH) by impacting the flow and pressure within the pulmonary vasculature, resulting in endothelial dysfunction and metabolic changes. A prudent line of treatment in this type of PH would be targeted therapy to relieve the pressure and reverse the flow-related changes. We used a swine model in order to mimic PH after PVS using pulmonary vein banding (PVB) of the lower lobes for 12 weeks to mimic the hemodynamic profile ass ...[more]

PMID: 36902298

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