High Field Asymmetric Waveform Ion Mobility Spectrometry- Mass Spectrometry to Enhance Cardiac Muscle Proteome Coverage
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Heart
SUBMITTER: 
Aleksandr Stotland
LAB HEAD: Jennifer Van Eyk
PROVIDER: PXD039174 | Pride | 2023-07-20
REPOSITORIES: Pride
Publications
High-Field Asymmetric Waveform Ion Mobility Spectrometry: Practical Alternative for Cardiac Proteome Sample Processing.
Ai Lizhuo L Binek Aleksandra A Kreimer Simion S Ayres Matthew M Stotland Aleksandr A Van Eyk Jennifer E JE
Journal of proteome research 20230411 6
Heart tissue sample preparation for mass spectrometry (MS) analysis that includes prefractionation reduces the cellular protein dynamic range and increases the relative abundance of nonsarcomeric proteins. We previously described "IN-Sequence" (IN-Seq) where heart tissue lysate is sequentially partitioned into three subcellular fractions to increase the proteome coverage more than a single direct tissue analysis by mass spectrometry. Here, we report an adaptation of the high-field asymmetric ion ...[more]
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