Proteomics

Dataset Information

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UBOX-UBL versus UBOX-UBLcontrol tranfected into HeLa Cells

ABSTRACT: In this work, we developed a suite of linked-domain UBE2D inhibitors. We transfected these constructs into HeLa cells and used shotgun proteomics with label-free quantification to measure which protein's abundances are affected by UBE2D inhibition.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cervical Epithelial Cell

DISEASE(S): Cervix Carcinoma

SUBMITTER: Joseph Harrison  

LAB HEAD: Joseph S.

PROVIDER: PXD040264 | Pride | 2025-12-11

REPOSITORIES: Pride

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UC1-2.raw Raw
UC1-3.raw Raw
UC1.raw Raw
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Publications

Ubiquitin (Ub) is a protein post-translational modifier that controls proteostasis through mechanisms spanning transcription, translation, and protein degradation. Ub conjugation occurs through a cascade of three enzyme classes (E1, E2, and E3s) involving >1,000 proteins that regulate the ubiquitination of cellular proteins. The E2 Ub-conjugating enzymes are the midpoint, yet their cellular roles remain under-characterized. Here, we develop highly selective and potent pan-UBE2D/UBCH5 inhibitors  ...[more]

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