Dataset Information

Proteome characterization of HCMV virus microenvironment with addition of cidofovir

ABSTRACT: The control of host processes which influence cell-cell communication is central to determining the outcome of a virus infection in tissue. Despite this, it remains unclear how cells either in close or distant proximity to an infected cell differentially respond to the primary infection. Here, we established a virus infection microenvironment to resolve molecular features and functional consequences of cell spatial address within this localized niche by mass spectrometry. To rule out the possibility that neighboring cells are infected by the viral progeny from the primary infection, cidofovir is added in the system to prevent viral genome replication in order to prevent the spread of infection.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Bokai Song

LAB HEAD: Ileana Cristea

PROVIDER: PXD040582 | Pride | 2023-05-11

REPOSITORIES: Pride

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Publications

Intercellular communication within the virus microenvironment affects the susceptibility of cells to secondary viral infections.

Song Bokai B Sheng Xinlei X Justice Joshua L JL Lum Krystal K KK Metzger Peter J PJ Cook Katelyn C KC Kostas James C JC Cristea Ileana M IM

Science advances 20230510 19

Communication between infected cells and cells in the surrounding tissue is a determinant of viral spread. However, it remains unclear how cells in close or distant proximity to an infected cell respond to primary or secondary infections. We establish a cell-based system to characterize a virus microenvironment, distinguishing infected, neighboring, and distal cells. Cell sorting, microscopy, proteomics, and cell cycle assays allow resolving cellular features and functional consequences of proxi ...[more]

PMID: 37163594

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Dataset Information

Proteome characterization of HCMV virus microenvironment with addition of cidofovir

Publications

Intercellular communication within the virus microenvironment affects the susceptibility of cells to secondary viral infections.

Similar Datasets

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