Proteomics analysis of preterm lung tissue with mass spectrometry to understand the impact of tidal volume strategy at birth

ABSTRACT: This study aimed to use a lamb model of preterm birth to investigate the impact of different tidal volume strategies, applied during positive pressure ventilation, on lung injury development. Preterm lambs were ventilated for a total of 15 minutes, followed by a 30 minute ‘rest’ period where lambs were supported via the intact placenta. A group of lambs was also euthanised at birth to act as an unventilated control group. At post-mortem lung tissue was sampled for proteomic analysis. Lung tissue samples were analysed by mass spectrometry-based proteomics using a TMT-11plex labelling approach, followed by nano-liquid chromatography coupled to an Q Exactive HF-X benchtop Orbitrap mass spectrometer in a data-dependent acquisition mode.

INSTRUMENT(S):

ORGANISM(S): Ovis Aries

TISSUE(S): Lung, Epithelial Cell

DISEASE(S): Mixed Disorder As Reaction To Stress

SUBMITTER: david greening  

LAB HEAD: David Greening & Prudence Pereira-Fantini

PROVIDER: PXD041917 | Pride | 2023-10-09

REPOSITORIES: Pride