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MRG15 interactome upon UV irradiation

ABSTRACT: The aim of this analysis is to identify the difference between interaction partners of MRG15 wild type and Chromodomain and MRG domain mutants upon UV irradiation. Therefore, MRG15 wild type and mutants (N-terminal FLAG tag) were overexpressed in U2OS cells. After UV irradiation, immunoprecipitation of the constructs was performed using FLAG M2 affinity gel. The constructs were eluted from the beads with 3xFLAG peptide.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Reihaneh Khaleghi

LAB HEAD: Hanspeter Naegeli

PROVIDER: PXD043089 | Pride | 2023-07-03

REPOSITORIES: Pride

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ASH1L-MRG15 methyltransferase deposits H3K4me3 and FACT for damage verification in nucleotide excision repair.

Maritz Corina C Khaleghi Reihaneh R Yancoskie Michelle N MN Diethelm Sarah S Brülisauer Sonja S Ferreira Natalia Santos NS Jiang Yang Y Sturla Shana J SJ Naegeli Hanspeter H

Nature communications 20230701 1

To recognize DNA adducts, nucleotide excision repair (NER) deploys the XPC sensor, which detects damage-induced helical distortions, followed by engagement of TFIIH for lesion verification. Accessory players ensure that this factor handover takes place in chromatin where DNA is tightly wrapped around histones. Here, we describe how the histone methyltransferase ASH1L, once activated by MRG15, helps XPC and TFIIH to navigate through chromatin and induce global-genome NER hotspots. Upon UV irradia ...[more]

PMID: 37393406

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