Proteomic profiling for elucidating the molecular mechanism in early seed development in rice
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ABSTRACT: Understanding the molecular mechanisms underlying early seed development is important for improving grain yield and quality in crop plants. To investigate the molecular mechanisms that occur during early seed development, we performed comparative label-free quantitative proteomic analysis on developing WT rice seeds between 0 and 1 day after pollination (DAP). A total of 5231 proteins were identified, and 902 proteins showed differential accumulation between 0 and 1 DAP seeds. Further analysis focused on the proteins preferentially expressed at 1 DAP and revealed an enrichment of proteins involved in DNA replication and pyrimidine biosynthetic pathways. Notably, the transcript of OsCTPS1, a cytidine triphosphate synthase known to play an essential role in early endosperm development, did not differ between 0 and 1 DAP, but OsCTPS1 protein accumulated more specifically at 1 DAP than at 0 DAP. We then found that inhibiting phosphorylation increased the stability of this protein. Furthermore, in osctps1-2, minichromosome maintenance (MCM) proteins were significantly reduced compared to WT at 1 DAP, and mutations in OsMCM5 caused defects in seed development. In this study, we tried to understand the molecular mechanisms of early seed development at the post-transcriptional regulation.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Oryza Sativa (rice)
TISSUE(S): Ovary
SUBMITTER: Sun Tae Kim
LAB HEAD: Sun Tae Kim
PROVIDER: PXD043162 | Pride | 2023-12-05
REPOSITORIES: Pride
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