Proteomics

Dataset Information

Deep Proteomic Compound Profiling with the Orbitrap Ascend Tribrid Mass Spectrometer Using Tandem Mass Tags and Real-Time Search

ABSTRACT: We evaluated the performance of the Orbitrap Ascend in comparison to the Orbitrap Eclipse in RTS-SPS-MS3-based analysis of human proteomes using fractionated and unfractionated samples labeled with TMT and TMTpro reagents. We did this comparison in the context of different sample input amounts; we analyzed differences in quantitative precision; we compared the effectiveness in identifying protein abundance changes induced by treatment with covalent inhibitors of PIN1.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: Steven Shuken  

LAB HEAD: Qing Yu

PROVIDER: PXD043660 | Pride | 2023-10-13

REPOSITORIES: Pride

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Action DRS
20220722_Eclipse_2ProteomeMix_Keese_qysrsColumn_HYpro_TMT16_G65min_HrMS2_HCD36_IT54_30K_phiSDM_028.raw Raw
20220722_Eclipse_2ProteomeMix_Keese_qysrsColumn_HYpro_TMT16_G65min_HrMS2_HCD36_IT86_027.raw Raw
20220722_Eclipse_2ProteomeMix_Keese_qysrsColumn_HYpro_TMT16_G65min_RTSMS3_CID34IT23MS2_HCD55MS3IT105ms_029.raw Raw
20220722_Eclipse_2ProteomeMix_Keese_qysrsColumn_HYpro_TMT16_G65min_RTSMS3_CID34IT23MS2_HCD55MS3IT54ms_30K_phiSDM_030.raw Raw
20220726_Ascend_2ProteomeMix_Keese_qysrsColumn_HYpro_TMT16_G65min_HrMS2_HCD36_IT54_30K_phiSDM_028.raw Raw
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