Proteomics

Dataset Information

0

Silver carp (Hypophthalmichthys molitrix) dorsal muscle LC-MSMS data


ABSTRACT: China produces more than 77.9% of the world's production of silver carp in 2020 with the production of 3812.9 kiloton.3 The high consumption of silver carp in China is mainly by using its edible muscles for manufacture of surimi-based seafoods or other muscle foods, which may contribute high quality protein resource and other valuable nutrients in human diets. This project is to understanding the muscle composition of slaughtered fish skeletal muscle use the proteomics methods. The proteomics was performed based on the improved DDA experiment with extensive fractionation and prolonged separation of peptides, and protein searching database was informed by the Iso-seq transcriptomics.

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Hypophthalmichthys Molitrix

TISSUE(S): White Muscle

SUBMITTER: Xidong Jiao  

LAB HEAD: Daming Fan, Xidong Jiao

PROVIDER: PXD043702 | Pride | 2024-01-26

REPOSITORIES: Pride

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Publications

Postmortem Muscle Proteome Characteristics of Silver Carp (<i>Hypophthalmichthys molitrix</i>): Insights from Full-Length Transcriptome and Deep 4D Label-Free Proteomic.

Jiao Xidong X   Li Xingying X   Zhang Nana N   Zhang Wenhai W   Yan Bowen B   Huang Jianlian J   Zhao Jianxin J   Zhang Hao H   Chen Wei W   Fan Daming D  

Journal of agricultural and food chemistry 20240102 2


The coverage of the protein database directly determines the results of shotgun proteomics. In this study, PacBio single-molecule real-time sequencing technology was performed on postmortem silver carp muscle transcripts. A total of 42.43 Gb clean data, 35,834 nonredundant transcripts, and 15,413 unigenes were obtained. In total, 99.32% of the unigenes were successfully annotated and assigned specific functions. PacBio long-read isoform sequencing (Iso-Seq) analysis can provide more accurate pro  ...[more]

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