Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Citrullinated proteins in monocyte plasma membrane fractions

ABSTRACT: Citrullinated proteins found in the plasma membrane fractions of monocytes incubated in calcium and EDTA

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Blood

SUBMITTER: Mekha Thomas

LAB HEAD: Erika Darrah

PROVIDER: PXD044370 | Pride | 2024-04-18

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Similar Datasets

Citrullinated proteins in monocyte organelle fractions

Project description:Citrullinated proteins found in the organelle fractions of monocytes incubated in calcium

2023-10-03 | PXD044188 | Pride

Screening of rheumatoid arthritis patients sera against citrullinated fibrinogen peptides using ImmunoCAP ISAC system

Project description:Citrullinated and unmodified peptides (>95% purity, ProImmune AB) were immobilized onto a chemically modified glass slide, sera from RA patients and healthy controls were applied into the reactions sites and fluorescence intensity after incubation with anti-human IgG antibody was acquired in a laser scanner. Final results for each citrullinated peptide were calculated by subtracting the intensity values of corresponding arginine containing control peptide from citrullinated peptide for all RA patients and controls.

2015-10-25 | E-MTAB-3606 | biostudies-arrayexpress

Distribution of histone-3-citrullinated sites in peri-infarct neurons on day 4 after ischemic stroke

Project description:To investigate distribution of histone-H3-citrullinated sites in peri-infarct neurons on day 4 after ischemic stroke and sham-operated mice, we performed cleavage under targets and tagmentation (CUT&Tag) assay for citrullinated histone H3 of peri-infarct neurons from Padi4-dificient or control mice .

2023-07-24 | GSE232171 | GEO

Effect of native or citrullinated histone peptides on gene expression of THP-1 cells

Project description:To examine the cellular effects of histones, we analyzed the RNA-seq data of THP-1 cells treated with native or citrullinated H2B-α1 peptide.

2022-07-21 | GSE205096 | GEO

Citrullinated glucose-regulated protein 78 is a candidate target for cancer immunotherapy

Project description:In this study we investigate the potential of targeting citrullinated GRP78 for cancer therapy. We select five peptides and show the identification CD4 T cell responses to one citrullinated GRP78 epitope that is restricted through HLA DP*0401 and HLA-DR*0101 alleles. This peptide is detected by mass spectrometry in B16 melanoma grown in vivo and citrulline specific CD4 responses to this epitope mediate efficient therapy of established B16 melanoma tumours in HHDII/DP4 transgenic mouse model. We demonstrate the existence of a repertoire of responses to the citrullinated GRP78 peptide in healthy individuals.

2022-11-29 | PXD037918 | Pride

Confident identification of citrullinated peptides from P gingivalis

Project description:Porphyromonas gingivalis is a key pathogen in the development of chronic periodontitis and has recently been linked to the development of rheumatoid arthritis and Alzheimer’s disease. In this project the outer membrane vesicles (OMV) from Pg have been analyzed using a two-dimensional HFBA-based separation system combined with LC-MS. Three strains from P. gingivalis was analyzed in order to elucidate their citrullinome: wild-type (WT), a mutant with the active site cysteine mutated to alanine (C351A), as well as a knock-out mutant of peptidyl arginine deiminase (ΔPPAD). For optimal and positive identification and validation of citrullinated peptides and proteins, high resolution mass spectrometers and strict MS criteria were utilized. This may have compromised the total number of identified citrullination, but increased the confidence of the validation. A new 2D separation system proved to increase the strength of validation, and along with the use of an in-house build program, Citrullia, we establish a fast and easy semi-automatic (manual) validation of citrullinated peptides. For WT we identified 78 citrullinated proteins having a total of 161 citrullination sites. For C351A a single citrullination site was found and no citrullinations was found for ΔPPAD.

2019-11-22 | PXD015701 | Pride

Mice with FIA develop anti-native and anti-in vitro citrullinated fibrinogen antibodies

Project description:Synovial antigen arrays were probed with 1:150 dilutions of plasma derived from SJL mice immunized with fibrinogen emulsified in CFA or with CFA alone. Autoantibody binding was detected with a Cy3-conjugated goat-anti-mouse IgG/M secondary antibody. SAM was applied to identify antigens with statistically significant differences in array reactivity between FIA and CFA control plasma (q < 0.01) obtained from mice before boosting. The SAM hits were subjected to hierarchical cluster analysis and are displayed as a heatmap. Synovial array profiling of FIA plasma demonstrated autoreactive B-cell responses against peptides representing native fibrinogen, and B-cell epitope spreading resulting in additional targeting of citrullinated fibrinogen in the samples obtained before boosting.

2009-09-12 | GSE18023 | GEO

Mice with FIA develop anti-native and anti-in vitro citrullinated fibrinogen antibodies

2010-05-05 | E-GEOD-18023 | biostudies-arrayexpress

SAMHD1 deacetylation by SIRT1 promotes DNA end resection by facilitating DNA binding at double-strand breaks

Project description:Sterile alpha motif and HD domain-containing protein 1 (SAMHD1) has a dNTPase-independent function in promoting DNA end resection to facilitate DNA double-strand break (DSB) repair by homologous recombination (HR); however, it is not known if upstream signaling events govern this activity. Here, we show that SAMHD1 is deacetylated by the SIRT1 sirtuin deacetylase, facilitating its binding with ssDNA at DSBs, to promote DNA end resection and HR. SIRT1 complexes with and deacetylates SAMHD1 at conserved lysine 354 (K354) specifically in response to DSBs. K354 deacetylation by SIRT1 promotes DNA end resection and HR but not SAMHD1 tetramerization or dNTPase activity. Mechanistically, K354 deacetylation by SIRT1 promotes SAMHD1 recruitment to DSBs and binding to ssDNA at DSBs, which in turn facilitates CtIP ssDNA binding, leading to promotion of genome integrity. Our findings define a mechanism governing the dNTPase-independent resection function of SAMHD1 by SIRT1 deacetylation in promoting HR and genome stability.

2023-03-11 | PXD031357 | Pride

Citrullinated epitopes identified on tumor MHC class II by peptide elution stimulate both regulatory and Th1 responses and require careful selection for optimal tumor therapy

Project description:In this manuscript, we outline the identification of citrullinated peptide epitopes presented on the surface of tumor cells that can be targeted for tumor therapy. Peptide elution from MHC is a useful tool in the identification of novel targets and has been used by many in immunotherapy strategies. Here we describe the identification of post-translationally modified citrullinated peptides through elution from MHC class II molecules.

2021-11-24 | PXD029029 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data