Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterisation of proteomic alterations in worker bees in response to amitraz treatment during summer to winter transition in Apis mellifera colonies

ABSTRACT: The application of treatments to reduce/control the number of Varroa destructor mites within in Apis mellifera colonies is common practice in apiculture. The effectiveness of the treatments in reducing/removing Varroa mites from colonies has been extensively studied, however, the effects of the treatments on the bees within the colonies is lacking. This work utilises proteomics to investigate how the presence of Apivar® strips within hives effects the worker bees. Samples of A. mellifera were isolated from colonies one week before Apivar® treatment and were sampled for 1, 3, 6 and 8 weeks post-treatment application. The results highlight two large proteomic shifts in workers between Control and Week 1 samples in comparison to Weeks 3, 6 and 8 samples. Cuticular proteins were decreased in abundance and translational proteins were increased in abundance in Weeks 3, 6 and 8 in comparison to Control and Week 1 samples. The proteomic shift in Week 8 in comparison to the other treatment samples was not as intense, with a lower number of increased translational proteins, indicating a reduction of treatment effect the longer it is within the hive. The work provides an initial insight into the effects Apivar® has on the honeybee population and how it may contribute to a stressful hive environment to a colony previously infested with Varroa mites.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Apis Mellifera (honeybee)

SUBMITTER: Rachel Ward

LAB HEAD: Kevin Kavanagh

PROVIDER: PXD044805 | Pride | 2025-07-01

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Apivar_Sample_MaxQuant_Search_Output.zip	Other
	Apivar_Search_List.txt	Txt
	Control_1.raw	Raw
	Control_2.raw	Raw
	Control_3.raw	Raw

Items per page:

1 - 5 of 22

Similar Datasets

Transcriptome of Varroa destructor life cycle

Project description:To study the underlying molecular mechanisms during the Varroa destructor life cycle, we carried out transcriptomic profiling of seven stages: young mites (collected from P8 to P9 brood cells), phoretic mites (collected on adult bees), arresting mites (collected in unsealed L5 brood cells), pre-laying mites (collected from sealed brood cells containing moving larva), laying mites (collected from sealed brood cells containing pre-pupae), post-laying mites (collected from capped brood cells containing purple-eye and white-body pupae P5), emerging mites (collected from P8 to P9 brood cells). In addition, we sampled non-reproducing mites (collected from P5 brood cells, but without offspring), males (collected from P8 to P9 brood cells), and phoretic mites artificially reared in cages with adult bees. This study was performed using Apis mellifera L. honey bee colonies naturally infested by Varroa destructor mites. Adult mites were collected from 4 unrelated colonies.

2018-04-11 | GSE100554 | GEO

Antennae transcriptomics of Varroa-hygienic and Nurse/Forager bees

Project description:The Varroa mite represents the main threat of honey bees (Apis mellifera). Bees from some colonies can limit the proliferation of this parasite by detecting and removing parasitized brood, such behavior is defined as Varroa sensitive Hygiene (VSH). This is an important issue for selecting colonies that can survive Varroa outbreaks. We therefore study the molecular meachnisms underlying this behavior by comparing the antennae transcriptomic profile of VSH and non-VSH bees. Those profiles were further compared to to the profiles of nurses and forager profiles involved in brood care and food collection, respectively.

2017-02-09 | GSE63613 | GEO

Diversity of populations of Deformed wing virus in the honeybee (Apis mellifera) pupae overtly infected by injection of DWV virus preparations from overtly and covertly infected honeybees to haemolymph.

Project description:Experiment was designed (i) to analyse the strain composition of Deformed wing virus (DWV) populations in covertly and overtly infected honeybees (Apis mellifera) from Varroa-free and Varroa-infested colonies, and (ii) to determine abundance of the DWV strains following direct injection of the DWV preparations from covertly and overtly infected bees to the bee pupae haemolymph in the absence of Varroa destructor mites. Experiment included isolation of DWV preparations from the following bees: covertly-infected bees from Varroa-free colony, covertly infected bees exposed orally to the Varroa-selected DWV strains, and the overtly infected Varroa-exposed bees. Honeybee pupae were experimentally injected with those DWV preparations and sampled 4 days post injection following development of overt DWV infection. A series of the DWV cDNA fragment covering complete DWV genomic RNA sequences were amplified by RT-PCR using RNA extracted from virus preparations and the injected pupae. The cDNA preparations were sequenced using next generation(Illumina HighSeq 2000) paired-end sequencing to obtain data on the DWV strain composition.

2013-09-01 | E-MTAB-1675 | biostudies-arrayexpress

Nutrigenomics in honey bees: the impact of pollen nutrients on bee health

Project description:We studied the molecular mechanisms underlying the impact of pollen nutrients on honey bee (Apis mellifera) health and how those nutrients improve resistance to parasites. Using digital gene expression, we determined the changes in gene expression induced by pollen intake in worker bees parasitized or not by the mites Varroa destructor, known for suppressing immunity and decreasing lifespan of bees.

2011-11-06 | GSE25161 | GEO

Effect of Deformed wing virus (DWV) and Varroa destructor (mite) on gene expression in honeybee (Apis mellifra) pupae

Project description:Experiment was designed to study the effect of Deformed wing virus (DWV) and the mite Varroa destructor on global gene expression using microarray transcriptional profiling in developing worker honeybee (Apis mellifera). Newly hatched bee larvae (day 3 of bee development) were transferred from a Varroa-free colony with low DWV levels to a Varroa-infested colony with high levels of DWV in bees and Varroa mites. All transferred larvae were receiving the DWV strains present in this Varroa-infested colony with the food delivered by the nurse bees until their capping (day 8). About half of these larvae were capped with Varroa mite and were subjected to the mite piercing and feeding on their haemolymph during pupal development until sampling at purple eye stage (day 14). Exposure to the mite piercing and feeding resulted in about 1000-fold increase of the DWV levels in the majority of the mite-exposed pupae compared to the control pupae and the pupae not exposed to Varroa mites.

2013-06-01 | E-MTAB-1285 | biostudies-arrayexpress

Nutrigenomics in honey bees: the impact of pollen nutrients on bee health

2011-11-06 | E-GEOD-25161 | biostudies-arrayexpress

Effects of Deformed wing virus infection and the mite Varroa destructor on siRNA and miRNA composition in the honeybee (Apis mellifera)

Project description:Experiment was designed to study the effect of Deformed wing virus (DWV) and the mite Varroa destructor on on siRNA and miRNA composition using high-throughput sequencing of small RNA in developing worker honeybee (Apis mellifera). Newly hatched bee larvae (day 3 of bee development) were transferred from a Varroa-free colony with low DWV levels to a Varroa-infested colony with high levels of DWV in bees and Varroa mites. All transferred larvae were receiving the DWV strains present in this Varroa-infested colony with the food delivered by the nurse bees until their capping (day 8). About half of these larvae were capped with Varroa mite and were subjected to the mite piercing and feeding on their haemolymph during pupal development until sampling at purple eye stage (day 14). Exposure to the mite piercing and feeding resulted in about 1000-fold increase of the DWV levels in the majority of the mite-exposed pupae compared to the control pupae and the pupae not exposed to Varroa mites.

2013-09-01 | E-MTAB-1671 | biostudies-arrayexpress

Honey bee: Apis mellifera syriaca

Project description:Apis mellifera syriaca is the native honeybee subspecies of Jordan and much of the Middle East. It expresses behavioral adaptations to a regional climate with very high temperatures, nectar dearth in summer, attacks of the Oriental wasp Vespa orientalis and in most cases it is resistant to varroa mites. The Thorax control sample of A. m. syriaca in this experiment was originally collected and stored since 2001 from Wadi Ben Hammad a remote valley in the southern region of Jordan. Using morphometric and Mitochondrial DNA markers it was proved that bees from this area had show higher similarity than other samples collected from the Middle East as represented by reference samples collected in 1952 by Brother Adam. The samples L1-L5 are collected from the National Center for Agricultural Research and Extension breading apiary which was originally established for the conservation of Apis mellifera syriaca. Goal was to use the genetic information in the breeding for varroa resistant bees and to determine the successfulness of this conservation program. Project funded by USAID-MERC grant number: TA-MOU-09-M29-075.

2016-02-01 | GSE43554 | GEO

Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. interaction reveals changes impairing the winter bee generation

Project description:Extensive annual losses of honey bees (Apis mellifera L.) represent a global problem for agriculture and biodiversity. The parasitic mite Varroa destructor in association with viral co-infections plays a key role in this phenomenon; however, the precise mechanisms are still unclear. We employed a unique combination of transcriptomic, proteomic, metabolomic, and functional analyses to elucidate the effects of Varroa parasitisation. We focused on complex differences between parasitised and unparasitised ten-days old honey bee workers collected from identical colonies before overwintering. Honey bees exposed to mite parasitation during their development revealed alterations in transcriptome and proteome related to immunity, oxidative stress, olfactory recognition, metabolism of sphingolipids and RNA regulatory mechanisms. Specifically, immune reactions and sphingolipids metabolism were strongly up-regulated in parasitised honey bees; whereas olfactory recognition and oxidative stress pathways were down-regulated compared to unparasitised bees. Additionally, the metabolomic analysis confirmed the depletion of nutrients, decreased energy stores and generally disrupted metabolism of parasitised workers, as previously reported. By virtue of comprehensive omics-based analysis, we define the key changes in the honey bee facing Varroa parasitism and suggest possible mechanisms underlying its detrimental effects. This study provides a theoretical basis for future efforts in efficient control strategies against Varroa mites.

2023-06-23 | PXD030369 | Pride

Exposure of Apis mellifera to anti-Varroa destructor formic acid treatment induces significant proteomic alterations.

Project description:The parasitic mite Varroa destructor is a major threat to the health and productivity of Apis mellifera hives and induces its effect by feeding on the fat body of larvae/pupae and transmitting viruses. Control of Varroa populations can be attempted using a variety of treatments and the effect of one of these (the formic acid containing - Mite Away Quick (MAQ) strips) on the proteome of A. mellifera was assessed in this work. Samples of A. mellifera were isolated from hives one week prior to MAQ treatment, during the week of treatment and for two weeks after end of treatment, proteins were extracted and analysed by label free quantitative proteomics. The results indicated that samples isolated during the week of treatment showed increased abundance of a range of cuticular proteins (+ 2.65 fold to + 6.64 fold) and decreased abundance of proteins that deal with xenobiotics (Cytochrome P450 subunits -11.38 fold to -2.16 fold) Interestingly some proteins associated with the oxidative phosphorylation pathway were increased in abundance (Cox5a and Cox5b) but others such as (Coxfa4) were decreased. The results presented here reveal that application of MAQ strips caused a dramatic disruption to the proteome of A. mellifera but the effect is transient and that by two weeks after the end of treatment the proteome has returned to resemble that of the untreated control. While MAQ strips are effective in reducing Varroa populations, the results presented here indicate they can adversely affect the proteome of A. mellifera and may contribute to the elevated stress in hives previously affected by Varroa parasitisation.

2022-11-21 | PXD026058 | Pride