Dataset Information

Optimized isolation of protein on chromatin (iPOC) strategy reveals the dynamics of chromatin composition upon inhibition of PI3K/AKT/mTOR signaling

ABSTRACT: Signaling pathways play critical roles in executing and controlling important biological processes within cells. The dysregulation of signaling pathways modulates chromatin modifications to regulate the epigenome, thereby contributing to tumorigenesis and metastasis Despite their cancer-related importance, current studies are limited to the investigation of the signaling cascade and how to shut this down in a cancer context. Yet the ultimate effect of PI3K/AKT/mTOR activation cascades on chromatin composition have not been previously investigated. To unravel DBP function upon different pathway inhibitors, in this study, we 1) optimized the iPOC strategy, 2) assessed both the specificity and sensitivity of the approach, and 3) performed comprehensive quantitative chromatin proteomic analysis to identify the dynamics of DNA-bound proteome upon treatment with PI3K, AKT or mTOR inhibitors.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Huiyu Wang

LAB HEAD: Jeroen Krijgsveld

PROVIDER: PXD045235 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20230122_FS1_GS_HW_iPOC_C18_20min_Beads.raw	Raw
	20230122_FS1_GS_HW_iPOC_C18_20min_SN.raw	Raw
	20230122_FS1_GS_HW_iPOC_C18_5min_Beads.raw	Raw
	20230122_FS1_GS_HW_iPOC_C18_5min_SN.raw	Raw
	20230122_FS1_GS_HW_iPOC_SP3_20min_Beads.raw	Raw

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Publications

Isolation of Proteins on Chromatin Reveals Signaling Pathway-Dependent Alterations in the DNA-Bound Proteome.

Wang Huiyu H Syed Azmal Ali AA Krijgsveld Jeroen J Sigismondo Gianluca G

Molecular & cellular proteomics : MCP 20250120 3

Signaling pathways often convergence on transcription factors and other DNA-binding proteins that regulate chromatin structure and gene expression, thereby governing a broad range of essential cellular functions. However, the repertoire of DNA-binding proteins is incompletely understood even for the best-characterized pathways. Here, we optimized a strategy for the isolation of Proteins on Chromatin (iPOC) exploiting tagged nucleoside analogs to label the DNA and capture associated proteins, thu ...[more]

PMID: 39842777

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