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Quantitative proteomic analysis identifies the unfolded protein response as a host pathway co-opted by ASFV to promote replication

ABSTRACT: African swine fever virus (ASFV) has caused severe consequences for the global pig industry. In this study, we conducted a multi-organ proteomic analysis using a 4D label-free quantitative proteomics approach and mapped the organ-specific proteomic landscape during ASFV infection. This work overcomes the limitations of most existing studies, which are primarily restricted to in vitro cell models, and providesprovide a more comprehensive understanding of ASFV infection and pathogenesis. Notably, the viral D117L protein is identified as a critical modulator of host cellular responses, directly subverting the unfolded protein response (UPR) pathway through specific interactions with host UPR-associated proteins. Collectively, our work lays the foundation for understanding the pathogenesis of ASFV, providing potential therapeutic strategies against African swine fever (ASF).

INSTRUMENT(S):

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Spleen, Lymph Node, Kidney

SUBMITTER: baohong Liu

LAB HEAD: Haixue Zheng

PROVIDER: PXD047161 | Pride | 2025-11-28

REPOSITORIES: Pride

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	FA254LQ_PA244_5_Slot2-34_1_1643.d.zip	Other
	FA254LQ_PA246_3_Slot2-31_1_1639.d.zip	Other
	FA254LQ_PA248_0_Slot2-28_1_1635.d.zip	Other
	FA254LQ_PA249_0_Slot2-29_1_1636.d.zip	Other
	FA254LQ_PA250_3_Slot2-32_1_1640.d.zip	Other

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