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DPP8/9 processing of human AK2 unmasks an IAP binding motif

ABSTRACT: AK2 is n-terminally processed by methionine aminopeptidases, N-terminal acetylation and DDP8/9 to become an active protein which is then sorted in the mitochondrial intermembrane space. We used peptide pull-downs of free and acetylated N-terminal peptides of AK2 from the different processing steps to identify binding partners by MS. We identified the inhibitor of apoptosis E3 ligase proteins (IAPs; BIRC2, 3, 6 and XIAP) as new binding partners for processed AK2 and show that they are responsible for AK2 degradation in the cytosol.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Tanja Bange

LAB HEAD: Tanja Bange

PROVIDER: PXD047178 | Pride | 2025-03-13

REPOSITORIES: Pride

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			Action	DRS
	20220317_Q2_U4_1-10_AB_SA_FM1052_10.raw	Raw
	20220317_Q2_U4_1-10_AB_SA_FM1052_12.raw	Raw
	20220317_Q2_U4_1-10_AB_SA_FM1052_14.raw	Raw
	20220317_Q2_U4_1-10_AB_SA_FM1052_16.raw	Raw
	20220317_Q2_U4_1-10_AB_SA_FM1052_18.raw	Raw

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