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Differences between static supernatant-, static biofilm- and flow biofilm-cells of two Lactiplantibacillus plantarum strains.

ABSTRACT: This study was conducted to analyze phenotypic and proteomic differences of two Lactiplantibacillus plantarum strains (WCFS1, model strain from human saliva, and CIP104448, stool isolate) when a biofilm was produced under static conditions (well researched), or with the addition of flow (novel).

INSTRUMENT(S):

ORGANISM(S): Lactiplantibacillus Sp. Lactobacillus Plantarum Wcfs1

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD047751 | Pride | 2024-06-03

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	MQresult_CIP_TA373-387.zip	Other
	MQresult_WCFS_TA370-384.zip	Other
	TA370_WCFS_S_Sup.raw	Raw
	TA371_WCFS_S_Bio.raw	Raw
	TA372_WCFS_F_Bio.raw	Raw

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1 - 5 of 21

Publications

Phenotypic and proteomic differences in biofilm formation of two <i>Lactiplantibacillus plantarum</i> strains in static and dynamic flow environments.

Huijboom Linda L Rashtchi Parisa P Tempelaars Marcel M Boeren Sjef S van der Linden Erik E Habibi Mehdi M Abee Tjakko T

Biofilm 20240422

<i>Lactiplantibacillus plantarum</i> is a Gram-positive non-motile bacterium capable of producing biofilms that contribute to the colonization of surfaces in a range of different environments. In this study, we compared two strains, WCFS1 and CIP104448, in their ability to produce biofilms in static and dynamic (flow) environments using an in-house designed flow setup. This flow setup enables us to impose a non-uniform flow velocity profile across the well. Biofilm formation occurred at the bott ...[more]

PMID: 38706985

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Food-associated Lactiplantibacillus plantarum C9O4 reverts inflammation and apoptosis in intestinal epithelial cells: a proteomic insight

Project description:Nowadays, Western diets and lifestyle lead to an increasing occurrence of chronic gut inflammation, that represents an emerging health concern with still a lack of successful therapies. Fermented foods, and their associated Lactic Acid Bacteria, have recently regained popularity for their probiotic potential including the maintenance of gut homeostasis by modulating the immune and inflammatory response. Our study aims to investigate the cross-talk between the food-borne strain Lactiplantibacillus plantarum C9O4 and intestinal epithelial cells in an in vitro inflammation model. Cytokines profile shows the ability of C9O4 to significantly reduce levels of IL-2, IL-5, IL-6, and IFN-γ. Proteomic functional analysis reveals an active host-microbe interaction that highlights an immunoregulatory role of C9O4, able to revert both the detrimental effects of IFN-γ through the JAK/STAT pathway and the apoptosis process in inflamed cells. These results suggest a promising therapeutic role of fermented food-associated microbes for the management of gastrointestinal inflammatory diseases.

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Identification of genes involved with P. aeruginosa biofilm antibiotic resistance by microarray analysis

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Project description:Identification of proteins contained in extracellular vesicles of Lactiplantibacillus plantarum PCM 2675. Dataset is related to publication http://dx.doi.org/10.20517/evcna.2024.49. This work was financially supported by the National Science Centre, Poland (no. 2021/43/D/NZ6/01464).

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GdpS contributes to Staphylococcus aureus biofilm formation by regulation of eDNA release

Project description:In Staphylococcus aureus, the role of the GGDEF domain containing protein GdpS remains poorly understood. Previous studies reported that gdpS mutant strains had decreased biofilm formation due to changes in icaADBC expression that were independent of cyclic-di-GMP levels. We deleted gdpS in three unrelated S. aureus isolates, and analyzed the resultant mutants for alterations in biofilm formation, metabolism and transcription. Dynamic imaging during biofilm development showed that GdpS inhibited early biofilm formation in only two out of the three strains examined, without affecting bacterial survival. However, quantification of biofilm formation using crystal violet staining revealed that inactivation of gdpS affected biofilm formation in all three studied strains. Extraction of metabolites from S. aureus cells confirmed the absence of cyclic-di-GMP, suggesting that biofilm formation in this species differs from that in other Gram-positive organisms. In addition, targeted mutagenesis demonstrated that the GGDEF domain was not required for GdpS activity. Transcriptomic analysis revealed that the vast majority of GGDEF-regulated genes were involved in virulence, metabolism, cell wall biogenesis and eDNA release. Finally, expression of lrgAB or deletion of cidABC in a strain lacking gdpS confirmed the role of GdpS on regulation of eDNA production that occurred without an increase in cell autolysis. In summary, S. aureus GdpS contributes to cell-to-cell interactions during early biofilm formation by influencing expression of lrgAB and cidABC mediated eDNA release. We conclude that GdpS acts as a negative regulator of eDNA release. Three strains UAMS-1, SA113 and SA564 were used in this study to compare wt with gdpS mutant after 5 hours of growth in static conditions (biofilm formation).

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Survey of microbiota composition differences between obese subjects before and after Lactiplantibacillus plantarum probiotic treatment

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Proteomic analysis of the exoproteome and surfaceome of Lactiplantibacillus plantarum CRL681: mechanisms of competitive exclusion against enterohemorrhagic Escherichia coli

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