Proteomic response of Staphylococcus aureus to bacteriophage infection. A systems approach to determining the response of Staphylococcus aureus to infection with Herelleviridae bacteriophage.

ABSTRACT: In the face of escalating challenges posed by infections with multiple drug-resistant (MDR) pathogens, the use of bacteriophages in therapeutic applications has emerged as a critically relevant and often sole alternative to traditional antibiotics. The most promising results of phage therapy have been demonstrated in the case of Staphylococcus aureus. It should be noted that the S. aureus population is characterized by a high degree of clonality. Strains belonging to sequence type 239 are among the main epidemic strains of methicillin-resistant S. aureus, identified worldwide. Many ST239 strains are characterized by MDR and resistance to virulent bacteriophages, and can become a serious problem in medicine. The purpose of this study was to investigate the resistance of clinical strains of S. aureus SA191REV of ST239 to bacteriophages of the Herelleviridae family using systems biology methods, starting from microbiological data on the interaction of strains with bacteriophages to transcriptomic and proteomic analysis. As results, the main changes in the cells of the resistant strain caused by phage infection are related to the energy metabolism in the cell. Significant changes were found in the functioning of the tricarboxylic acid cycle, glycolytic process, and glucose metabolic process. The most likely mechanism of resistance in the case of SA191REV strains is the premature death of infected cells due to the type I toxin-antitoxin system, as well as the hyperexpression of the lrgA and lrgB genes.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Staphylococcus Aureus

TISSUE(S): Cell Culture

SUBMITTER: Georgij Arapidi  

LAB HEAD: Georgij Arapidi

PROVIDER: PXD047971 | Pride | 2025-05-06

REPOSITORIES: Pride