Proteomics

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SWATH-MS insights on butyric acid effect on mAbs production and redox homeostasis in CHO cells.

ABSTRACT: Sodium butyrate (NaBu) is well-known for its capacity to hinder cellular growth and act as a histone deacetylase inhibitor. It is commonly employed in the cultivation of recombinant Chinese hamster ovary (CHO) cell cultures to boost the production of specific proteins, such as antibodies. In this investigation, two types of CHO cell lines, namely K1 and DG44, along with their respective mAb-producing lines, K1-Pr and DG44_Pr, were cultured with or without NaBu. To analyze the proteome, a SWATH-based profiling method was utilized. The outcomes were assessed using Spectronaut 17, while STRING and Gene Ontology pathway analyses were performed using Cytoscape. The analysis confirms the known effects of NaBu on mAbs production, adding information on redox homeostasis of the cells.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Mauro Galli  

LAB HEAD: Xuezhi Bi

PROVIDER: PXD048575 | Pride | 2025-05-06

REPOSITORIES: Pride

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20170725_RD_SWATH_CHOK1_CTRL1.wiff Wiff
20170725_RD_SWATH_CHOK1_CTRL1.wiff.scan Wiff
20170725_RD_SWATH_CHOK1_CTRL2.wiff Wiff
20170725_RD_SWATH_CHOK1_CTRL2.wiff.scan Wiff
20170725_RD_SWATH_CHOK1_CTRL3.wiff Wiff
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SWATH-MS insights on sodium butyrate effect on mAbs production and redox homeostasis in CHO cells.

Galli Mauro M   Liu Lillian Chia-Yi LC   Sim Kae Hwan KH   Kok Yee Jiun YJ   Wongtrakul-Kish Katherine K   Nguyen-Khuong Terry T   Tate Stephen S   Bi Xuezhi X  

AMB Express 20241224 1

Sodium butyrate (NaBu), well-known as a histone deacetylase inhibitor and for its capacity to impede cell growth, can enhance the production of a specific protein, such as an antibody, in recombinant Chinese hamster ovary (CHO) cell cultures. In this study, two CHO cell lines, namely K1 and DG44, along with their corresponding mAb-producing lines, K1-Pr and DG44-Pr, were cultivated with or without NaBu. A SWATH-based profiling method was employed to analyze the proteome. Cells cultured in the pr  ...[more]

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