Dataset Information

High transcriptional and proteomic abundance of the bromoform-synthesizing halogenase in seaweed Asparagopsis taxiformis revealed by a multi-omics investigation

ABSTRACT: Halogenases, and the halogenated organic molecules that they furnish are thought to be highly specialized secondary metabolites produced in only modest amounts. The widespread production of bromoform by marine macroalgae contradicts this paradigm wherein up to 8% dry weight of the red seaweed Asparagopsis taxiformis comprises of this brominated natural product. In this study, using paired transcriptomic and proteomic workflows, we determine that the bromoform-producing halogenase in A. taxiformis, Mbb1, is one of the most abundant proteins in the seaweed tissue with proteomic abundance rivaling that of enzymes involved in photosynthesis and carbon fixation. The proteomic abundance of Mbb1 is matched by the high transcript abundance of the mbb1 gene. Production of Mbb1 was found independent of the ecological source of the seaweed tissue with comparable transcript abundances detected between stress-free laboratory-cultivated, and appropriately stressed field-collected tissue samples. Taken together, these findings allow us to posit that bromoform production is not a stress-response or self-defense mechanism for A. taxiformis and instead fulfills a core metabolic role in marine macroalgal physiology.

INSTRUMENT(S):

ORGANISM(S): Asparagopsis Taxiformis

TISSUE(S): Plant Cell, Shoot

DISEASE(S): Disease Free

SUBMITTER: Shahima Islam

LAB HEAD: Vinayak Agarwal

PROVIDER: PXD052087 | Pride | 2026-02-16

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 17

Publications

Detection of unusually high transcriptomic and proteomic abundance of bromoform-synthesizing halogenase in marine macroalgae Asparagopsis taxiformis.

Lin Zhenjian Z Islam Shahima S Hargrave Matthew S MS Singh Rakesh R Zhou Lingjie L Xie Ramon R Smith Jennifer E JE Schmidt Eric W EW Agarwal Vinayak V

Frontiers in Marine Science 20251006

Halogenated molecules produced by marine algae are thought to be defensive secondary metabolites. The extraordinarily high concentration of bromoform in the seaweed Asparagopsis-up to 8% dry tissue weight-challenges the exclusivity of this paradigm. In this report, we provide evidence that the mbb1 gene which encodes the bromoform producing halogenase is among the most highly transcribed genes in Asparagopsis tissue, with the resulting Mbb1 protein abundance rivaling that of ...[more]

PMID: 41684644

Similar Datasets

Project description:Oxidative Stress Protection and the Repair Response To Hydrogen Peroxide in the Hyperthermophilic Archaeon Pyrococcus furiosus Pyrococcus furiosus is a shallow marine, anaerobic archaeon that grows optimally at 100Â°C. Addition of H2O2 (0.5 mM) to a growing culture resulted in cessation of growth with a 2 hour lag before normal growth resumed. Whole genome transcriptional profiling revealed that the main response occurs within 30 min of peroxide addition, with the up-regulation of 62 open reading frames (ORFs), 36 of which are part of 10 potential operons. More than half of the up-regulated ORFs are of unknown function while some others encode proteins that are involved potentially in sequestering iron and sulfide, in DNA repair and in generating NADPH. This response is thought to involve primarily damage repair rather than protection, since cultures exposed to sub-toxic levels of H2O2 were not more resistant to the subsequent addition of H2O2 (0.5 â 5.0 mM). Consequently, there is little if any induced protective response to peroxide, rather, the organism maintains a constitutive protective mechanism involving high levels of oxidoreductase-type enzymes such as superoxide reductase, rubrerythrin and alkyl hydroperoxide reductase I. The related hyperthermophiles P. woesei and Thermococcus kodakaraensis were more sensitive to peroxide than P. furiosus, apparently due to the lack of several of its peroxide-responsive ORFs. Pyrococcus furiosus (DSM 3638) was grown at 95Â°C in a 20-liter fermentor using maltose as the carbon and energy source. An exponential-phase culture of P. furiosus that had undergone three successive transfers in the experimental medium was used to inoculate the 20-liter fermentor. The culture was shocked with 0.5 mM of hydrogen peroxide (H2O2) when cell density was in mid-exponential phase (~ 5.0 Â´ 107 cells/ml, as determined by direct microscopic cell count). To obtain RNA for microarray and for quantitative PCR (QPCR) analyses, samples (2 liter) were rapidly removed from the fermentor and cooled to 4Â°C. Total RNA was extracted using acid-phenol and stored at -80Â°C until needed. A total of 3 biological replicates in triplicate (3 copies on the same slide) was used in the data set.

			Action	DRS
	20230818_SI_1F1.raw	Raw
	20230818_SI_1F2.raw	Raw
	20230818_SI_1F3.raw	Raw
	20230818_SI_1F4.raw	Raw
	20230818_SI_2F1.raw	Raw

Dataset Information

High transcriptional and proteomic abundance of the bromoform-synthesizing halogenase in seaweed Asparagopsis taxiformis revealed by a multi-omics investigation

Dataset's files

Publications

Detection of unusually high transcriptomic and proteomic abundance of bromoform-synthesizing halogenase in marine macroalgae <i>Asparagopsis taxiformis</i>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

High transcriptional and proteomic abundance of the bromoform-synthesizing halogenase in seaweed Asparagopsis taxiformis revealed by a multi-omics investigation

Dataset's files

Publications

Detection of unusually high transcriptomic and proteomic abundance of bromoform-synthesizing halogenase in marine macroalgae &lt;i&gt;Asparagopsis taxiformis&lt;/i&gt;.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Detection of unusually high transcriptomic and proteomic abundance of bromoform-synthesizing halogenase in marine macroalgae <i>Asparagopsis taxiformis</i>.