Dataset Information

PDZD8-FKBP8 tethering complex at ER-mitochondria contact sites regulates mitochondrial complexity

ABSTRACT: Proximity labeling-Mass spectrometry for PDZD8-TurboID KI Neuro2a cells

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Permanent Cell Line Cell, Cell Culture, Forebrain Neuroblast

DISEASE(S): Alzheimer's Disease

SUBMITTER: Chulhwan (Sean) Kwak

LAB HEAD: Yusuke Hirabayashi

PROVIDER: PXD052694 | Pride | 2025-05-07

REPOSITORIES: Pride

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Publications

Mitochondrial complexity is regulated at ER-mitochondria contact sites via PDZD8-FKBP8 tethering.

Nakamura Koki K Aoyama-Ishiwatari Saeko S Nagao Takahiro T Paaran Mohammadreza M Obara Christopher J CJ Sakurai-Saito Yui Y Johnston Jake J Du Yudan Y Suga Shogo S Tsuboi Masafumi M Nakakido Makoto M Tsumoto Kouhei K Kishi Yusuke Y Gotoh Yukiko Y Kwak Chulhwan C Rhee Hyun-Woo HW Seo Jeong Kon JK Kosako Hidetaka H Potter Clint C Carragher Bridget B Lippincott-Schwartz Jennifer J Polleux Franck F Hirabayashi Yusuke Y

Nature communications 20250417 1

Mitochondria-ER membrane contact sites (MERCS) represent a fundamental ultrastructural feature underlying unique biochemistry and physiology in eukaryotic cells. The ER protein PDZD8 is required for the formation of MERCS in many cell types, however, its tethering partner on the outer mitochondrial membrane (OMM) is currently unknown. Here we identify the OMM protein FKBP8 as the tethering partner of PDZD8 using a combination of unbiased proximity proteomics, CRISPR-Cas9 endogenous protein taggi ...[more]

PMID: 40246839

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