Dataset Information

Comparative quantitative proteomic analysis of Chlamydomonas reinhardtii cells from wild-type and chimeric FNR-PSAF mutant cells

ABSTRACT: Photosynthetic linear electron transfer (LET) comprises a solar energy driven pathway tha directs electrons to the production of NADPH, via ferredoxin-NADP+ reductase (FNR), while generating a proton motive force (pmf) to drive ATP synthesis. Both NADPH and ATP are consumed for carbon fixation. However, this pathway can be altered to block NADPH formation while maintaining pmf to produce ATP to enable acclimation of the organism to stress conditions at the expense of carbon fixation. LET and the alternative pathway, cyclic electron transfer (CET) both rely on protein-protein interactions with FNR but creating a knock-out mutant to explore its contribution to LET/CET regulation would be problematic given that FNR is essential. Instead, this project employs two mutants expressing a chimeric FNR-PSAF protein created by CRISPR-Cas9 gene editing: (1) NT12 = fnr-psaf/fnr/Δpsaf and (2) T7 = fnr-psaf/Δfnr/Δpsaf. The effects of these mutants, compared to wild-type cells, on the abundance of selected photosynthesis-related proteins are determined by MS-based quantitative proteomic analysis.

INSTRUMENT(S):

ORGANISM(S): Chlamydomonas Reinhardtii

TISSUE(S): Photosynthetic Cell

SUBMITTER: Philip Jackson

LAB HEAD: Professor Matthew Johnson

PROVIDER: PXD052753 | Pride | 2025-05-07

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	2024-04-25_PhilJackson_N01_01.raw	Raw
	2024-04-25_PhilJackson_N01_02.raw	Raw
	2024-04-25_PhilJackson_N01_03.raw	Raw
	2024-04-25_PhilJackson_N02_01.raw	Raw
	2024-04-25_PhilJackson_N02_02.raw	Raw

Items per page:

1 - 5 of 39

Publications

Tethering ferredoxin-NADP+ reductase to photosystem I promotes photosynthetic cyclic electron transfer.

Emrich-Mills Tom Z TZ Proctor Matthew S MS Degen Gustaf E GE Jackson Philip J PJ Richardson Katherine H KH Hawkings Frederick R FR Buchert Felix F Hitchcock Andrew A Hunter C Neil CN Mackinder Luke C M LCM Hippler Michael M Johnson Matthew P MP

The Plant cell 20250301 3

Fixing CO2 via photosynthesis requires ATP and NADPH, which can be generated through linear electron transfer (LET). However, depending on the environmental conditions, additional ATP may be required to fix CO2, which can be generated by cyclic electron transfer (CET). How the balance between LET and CET is determined remains largely unknown. Ferredoxin-NADP+ reductase (FNR) may act as the switch between LET and CET, channeling photosynthetic electrons to LET when it is bound to photosystem I (P ...[more]

PMID: 40037377

Similar Datasets

Project description:Even though mutations in epigenetic regulators frequently occur in myeloproliferative neoplasms, their effects on the epigenome have not been well studied. Furthermore, even though primary myelofibrosis (PMF) has a markedly worse prognosis compared to essential thrombocytosis (ET) or polycythemia vera (PV), the molecular distinctions between these subgroups are not well elucidated. We performed the HELP (HpaII tiny fragment enriched by LM-PCR) assay to study genome-wide methylation in PV, ET and PMF samples compared with healthy controls. We determined that PV and ET are characterized by aberrant promoter hypermethylation while PMF is an epigenetically distinct subgroup characterized by both aberrant hyper and hypomethylation. Aberrant hypomethylation in PMF was seen to occur in non CpG island loci, demonstrating further qualitative differences between the disease subgroups. The differentially methylated genes in PV and ET were involved predominantly in cell signaling pathways and were enriched for binding sites of GATA1 and other transcription factors. In contrast, aberrantly methylated genes in PMF were involved in inflammatory pathways and were enriched for NF1 (NFI), LEF1 and other transcription factors. Within the PMF subgroup, cases with ASXL1 disruptions formed an epigenetically distinct subgroup with relatively increased methylation. Cases of MPNs with TET2 mutations demonstrated decreased levels of hydroxymethylation and distinct set of hypermethylated genes. In contrast, the JAK2V617F mutation did not drive epigenetic clustering within MPNs. Finally, the significance of aberrant methylation was demonstrated by sensitivity of MPN derived cell lines to decitabine. These results demonstrate epigenetic differences between PMF and PV/ET and reveal methylomic signatures of ASXL1 and TET2 mutations. The study population consisted of 26 MPN patients (6 cases of ET, 8 cases of PV and 12 cases of PMF) and 3 healthy controls. Individual HpaII restriction digest profiles were compared to an internal MspI digest control, to yield differentially methylated fragments for every sample. The purpose of this study was to assess genome wide patterns of DNA methylation across the MPN stratified by disease class.