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Linear Protein Identification of Soluble and Insoluble Fractions from In-Situ DSSO Crosslinked Human Mitochondria

ABSTRACT: In this project, freshly isolated mitochondria from K-562 cells were crosslinked immediately with the homobifunctional and membrane-permeable reagent disuccinimidyl sulfoxide (DSSO). The study focuses on the comprehensive identification of proteins, specifically distinguishing between soluble and insoluble fractions. Both fractions were digested using Trypsin and analyzed using Liquid Chromatography coupled with Tandem Mass Spectrometry (LC-MS/MS) and Data-Dependent Acquisition (DDA) methods. The resulting data were processed using MaxQuant software.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Fabian Schildhauer

LAB HEAD: Juri Rappsilber

PROVIDER: PXD052817 | Pride | 2025-05-06

REPOSITORIES: Pride

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			Action	DRS
	B190809_02_HF_PR_IN_160_mito_DSSO_SOL_ID_rep1.mgf	Mgf
	B190809_02_HF_PR_IN_160_mito_DSSO_SOL_ID_rep1.raw	Raw
	B190809_03_HF_PR_IN_160_mito_DSSO_SOL_ID_rep2.mgf	Mgf
	B190809_03_HF_PR_IN_160_mito_DSSO_SOL_ID_rep2.raw	Raw
	B190809_04_HF_PR_IN_160_mito_DSSO_SOL_ID_rep3.raw	Raw

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