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Crosslinking Interaction Network of Human Mitochondria

ABSTRACT: Isolated human mitochondria underwent DSSO crosslinking. Following treatment, the mitochondria were separated into soluble and insoluble fractions. Both fractions were then subjected to multidimensional fractionation, including SCX and hSAX. The fractionated peptides were subsequently analyzed using CL-MS. FDR-based error estimation facilitated the creation of a comprehensive mitochondrial interaction network, revealing novel protein-protein interactions.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Fabian Schildhauer

LAB HEAD: Juri Rappsilber

PROVIDER: PXD052832 | Pride | 2025-05-06

REPOSITORIES: Pride

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			Action	DRS
	B190814_02_HF_PR_IN_130_mito_DSSO_INSOL_SCX16_hSAX06_rep1_LSmeth.raw	Raw
	B190814_02_HF_PR_IN_130_mito_DSSO_INSOL_SCX16_hSAX06_rep1_oldmeth.raw	Raw
	B190814_03_HF_PR_IN_130_mito_DSSO_INSOL_SCX16_hSAX06_rep2_LSmeth.raw	Raw
	B190814_03_HF_PR_IN_130_mito_DSSO_INSOL_SCX16_hSAX06_rep2_oldmeth.raw	Raw
	B190814_04_HF_PR_IN_130_mito_DSSO_INSOL_SCX16_hSAX06_rep3_LSmeth.raw	Raw

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