Proteomics

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Human colorectal cancer cell lines with manipulated AGR2 expression SWATH MS

ABSTRACT: SWATH MS analysis of colorectal adenocarcinoma derived paired cellular model, namely SW480 derived from a primary tumour and SW620 derived from the tumour´s lymph node metastasis. The whole proteome spectra were analysed to assess the changes caused by the manipulation of AGR2 expression. AGR2, a protein disulphide isomerase, is often overexpressed in tumours and connected with proliferation, survival, chemoresistance, and metastasis. Parental SW480 is AGR2 negative, so a stable expressing clone was prepared, while SW620 is endogenously positive for AGR2; therefore, two CRISPR/Cas9 mediated knockout clones were generated. The SWATH MS analysis generated a set of differentially expressed proteins with respect to AGR2 expression. This allowed us to identify new potential regulatory mechanisms and molecular pathways in which the AGR2 might be involved.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Colon

DISEASE(S): Colorectal Adenocarcinoma

SUBMITTER: Jakub Faktor  

LAB HEAD: Lenka Hernychova

PROVIDER: PXD053085 | Pride | 2025-04-23

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
2021-12-21-decoys-contam-Human_TrEMBL_SPROT.FASTA.fas Fasta
EXP1_AND_EXP3_DDA_spectral_library_1.mzML Mzml
EXP1_AND_EXP3_DDA_spectral_library_1_FRAGGER.pep.xml Pepxml
EXP1_AND_EXP3_DDA_spectral_library_2.mzML Mzml
EXP1_AND_EXP3_DDA_spectral_library_2_FRAGGER.pep.xml Pepxml
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Publications

Characterization of the AGR2-NPM3 axis uncovers the AGR2 involvement in PD-L1 regulation in colorectal cancer.

Martisova Andrea A   Faktor Jakub J   Sosolikova Tereza T   Klemesova Iveta I   Kolarova Tamara T   Holcakova Jitka J   Hrstka Roman R  

Scientific reports 20240920 1

Despite extensive research, the molecular role of AGR2 in the progression and metastasis of colorectal cancer (CRC) has not been fully characterized. We used quantitative mass spectrometry (SWATH MS) to identify differentially expressed proteins in paired CRC cell models of the SW480 and SW620 cell lines in response to AGR2 protein level manipulation. Relying on the results from SWATH MS and subsequent immunochemical validation, we selected NMP3 as the top candidate protein associated with AGR2  ...[more]

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