Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MS analysis of subcellular compartment of hiPSC-derived motor neurons

ABSTRACT: Local proteomes of axonal and somatodendritic compartments of hiPSC-derived motor neurons and FUSR244RR-ALS model.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Christian Sommer

LAB HEAD: Prof. Dr. Matthias Selbach

PROVIDER: PXD054876 | Pride | 2025-11-24

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Gonzo_20200422_TM_HS_MotoNeuron_Mn1.raw	Raw
	Gonzo_20200422_TM_HS_MotoNeuron_Mn10.raw	Raw
	Gonzo_20200422_TM_HS_MotoNeuron_Mn11.raw	Raw
	Gonzo_20200422_TM_HS_MotoNeuron_Mn12.raw	Raw
	Gonzo_20200422_TM_HS_MotoNeuron_Mn13.raw	Raw

Items per page:

1 - 5 of 28

Publications

Neuromuscular dysfunction in patient-derived FUS<sup>R244RR</sup>-ALS iPSC model via axonal downregulation of neuromuscular junction proteins.

von Kügelgen Nicolai N Ludwik Katarzyna K Mendonsa Samantha S Römer Christine C Becher Erik E Breimann Laura L Strauch Mara M Mari Tommaso T Mongellaz Sandrine S Zuckerman Binyamin B Efendic Fatima F Grexa Nina N Oliveras-Martinez Anna A Woehler Andrew A Selbach Matthias M La Bella Vincenzo V Ulitsky Igor I Chekulaeva Marina M

NAR molecular medicine 20250312 2

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative condition characterized by the progressive degeneration of motor neurons (MNs), ultimately resulting in death due to respiratory failure. A common feature among ALS cases is the early loss of axons, pointing to defects in axonal transport and translation as initial disease indicators. ALS is associated with mutations in RNA-binding proteins, such as FUS (Fused in Sarcoma). Here, we established a FUS<sup>R244RR</sup>-ALS hiPSC-derived mod ...[more]

PMID: 41255704

Similar Datasets

Differentiation of human induced pluripotent stem cells into induced motor neurons

Project description:Characterisation of induced motor neurons (iMN) generated from human induced pluripotent stem cells (hiPSC, hPSCreg name BIHi005-A) using doxycycline inducible expression of Ngn2, Isl1 and Lhx3. While this method of iMN generation has been described before (REF: doi.org/10.1002/cpcb.51), no extensive characterisation of the transcriptome and proteome throughout the differentiation process or of the mature neurons has been undertaken so far.

2025-11-24 | PXD031066 | Pride

hiPSC-derived motor neurons: sporadic ALS patients vs. controls

Project description:Differentiated motor neurons from hiPSC derived from peripheral nerve fibroblasts of sporadic ALS patients and evaluated the gene expression profile by means microarray-linked to specific analysis tools. Two-condition experiment, ALS patients motor neurons vs. controls. Biological replicates: 3 ALS replicates, 3 control replicates.

2015-04-25 | E-GEOD-68240 | biostudies-arrayexpress

scRNAseq in human iPSC-derived motor neurons(hiPSC-MNs).

Project description:We sequenced mRNA from hiPSC-derived human motor neurons to confirm gene expression in these cells.

2024-09-27 | GSE267791 | GEO

Subcellular whole transcriptome profiling reveals the RNA composition of motor axons

Project description:Most RNAs within polarized cells such as neurons are sorted subcellularly in a coordinated manner. Despite advances in the development of methods for profiling polyadenylated RNAs from small amounts of input RNA, techniques for profiling coding and non-coding RNAs simultaneously are not well established. Here, we optimized a transcriptome profiling method based on double-random priming and applied it to serially diluted total RNA down to 10 pg. Read counts of expressed genes were robustly correlated between replicates, indicating that the method is both reproducible and scalable. Our transcriptome profiling method detected both coding and long non-coding RNAs sized >300 bases. Compared to total RNA-seq using a conventional approach, our protocol detected 70% more genes due to reduced capture of ribosomal RNAs. We used our method to analyze the RNA composition of compartmentalized motoneurons. The somatodendritic compartment was enriched for transcripts with post-synaptic functions as well as for certain nuclear non-coding RNAs such as 7SK. In axons, transcripts related to translation were enriched including the cytoplasmic non-coding RNA 7SL. Our profiling method can be applied to a wide range of investigations including perturbations of subcellular transcriptomes in neurodegenerative diseases, and investigations of microdissected tissue samples such as anatomically defined fiber tracts.

2015-10-13 | GSE66230 | GEO

mRNAseq in control and siQKI in human iPSC-derived motor neurons(hiPSC-MNs).

Project description:We sequenced mRNA from hiPSC-MNs to compare gene expression level and alternative splicing events between 3 control and 3 siQKI.

2024-09-27 | GSE218824 | GEO

Investigating hiPSC-CMs_DongLi

Project description:Proteomic analysis of hiPSC-CMs with drug treatment

2021-05-01 | MSV000087351 | MassIVE

scRNAseq in human iPSC-derived motor neurons(hiPSC-MNs).

Project description:scRNAseq in human iPSC-derived motor neurons(hiPSC-MNs).

| PRJNA1112886 | ENA

Single-cell RNA sequencing of human liver development and hiPSC models

Project description:scRNA-seq of primary human adult liver, primary human fetal liver (5 post conceptional weeks), EPCAM+ MACS sorted primary human intrahepatic duct cholangiocytes, sequential timepoints of hiPSC-derived hepatocyte-like cells (HLC), sequential timepoints of hiPSC-derived cholangiocyte-like cells (CLC), sequential timepoints of hiPSC-derived hepatic stellate-like cells (HSLC), sequential timepoints of hiPSC-derived endothelial-like cells (ELC), sequential timepoints of hiPSC-derived macrophage-like cells (MLC) and lentiviral transduced hiPSC-derived hepatocyte-like cells.

2022-05-04 | E-MTAB-8210 | biostudies-arrayexpress

Subcellular transcriptome alterations in a cell culture model of spinal muscular atrophy point to widespread defects in axonal growth and presynaptic differentiation

Project description:Neuronal function critically depends on coordinated subcellular distribution of mRNAs. Disturbed mRNA processing and axonal transport has been found in spinal muscular atrophy and could be causative for dysfunction and degeneration of motoneurons. Despite the advances made in characterizing the transport mechanisms of several axonal mRNAs, an unbiased approach to identify the axonal repertoire of mRNAs in healthy and degenerating motoneurons has been lacking. Here we used compartmentalized microfluidic chambers to investigate the somatodendritic and axonal mRNA content of cultured motoneurons by microarray analysis. In axons, transcripts related to protein synthesis and energy production were enriched relative to the somatodendritic compartment. Knockdown of Smn, the protein deficient in spinal muscular atrophy, produced a large number of transcript alterations in both compartments. Transcripts related to immune functions, including MHC class I genes, and with roles in RNA splicing were upregulated in the somatodendritic compartment. On the axonal side, transcripts associated with axon growth and synaptic activity were downregulated. These alterations provide evidence that subcellular localization of transcripts with axonal functions as well as regulation of specific transcripts with nonautonomous functions is disturbed in Smn-deficient motoneurons, most likely contributing to the pathophysiology of spinal muscular atrophy.

2014-12-01 | GSE59506 | GEO

Comparing Control and Schizophrenic hiPSC-derived Neurons

Project description:Schizophrenia is a debilitating neurological disorder for which no cure exists. Few defining characteristics of schizophrenic neurons have been identified and the molecular mechanisms responsible for schizophrenia are not well understood, in part due to the lack of patient material for study. Human induced pluripotent stem cells (hiPSCs) offer a new strategy for studying schizophrenia. We have created the first cell-based human model of a complex genetic psychiatric disease by generating hiPSCs from schizophrenic patients and subsequently differentiating these cells to hiPSC-derived neurons in vitro. Schizophrenic hiPSC-derived neurons showed diminished neuronal connectivity in conjunction with decreased neurite number, PSD95-protein levels and glutamate receptor expression. Gene expression profiles of schizophrenic hiPSC-derived neurons identified altered expression of many components of the cAMP and WNT signaling pathways. Key cellular and molecular elements of the schizophrenic phenotype were ameliorated following treatment of schizophrenic hiPSC-derived neurons with the antipsychotic loxapine. 3 independent differentiations (biological replicates) for each of four control and four schizophrenic patients were analyzed.

2011-05-01 | E-GEOD-25673 | biostudies-arrayexpress