Dataset Information

I.e., Human HEK-293F cell line AP-MS/MS analysis of oxidative modifications in SPATA5.

ABSTRACT: The objective of this project was to identify oxidative modifications of the SPATA5 protein in C672 cells through proteomic analysis. This involved the affinity purification of SPATA5 under overexpression conditions, followed by detailed proteomic analysis to characterize the oxidative modifications.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Yuhao Dai

LAB HEAD: Ning Gao

PROVIDER: PXD054917 | Pride | 2025-05-07

REPOSITORIES: Pride

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Publications

Cryo-EM structure of the AAA+ SPATA5 complex and its role in human cytoplasmic pre-60S maturation.

Dai Yuhao Y Wu Damu D Li Ningning N Ma Chengying C Zhang Yunyang Y Gao Ning N

Nature communications 20250423 1

Eukaryotic ribosome biogenesis is an energy-consuming process involving many ATPase-driven steps. In yeast, AAA+ protein Drg1 releases an assembly factor Rlp24, a placeholder for Rpl24, from pre-60S particles just exported to cytosol. The equivalent process in human cells involves SPATA5 (Drg1 homolog) and additional factors. However, the mechanistic details remain unclear. Here we reveal that SPATA5 forms a 4:2:2:2 complex with SPATA5L1, C1orf109, and CINP. This complex features an N-terminal r ...[more]

PMID: 40268917

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			Action	DRS
	DYH_20240607_5_EQ.mzML	Mzml
	DYH_20240607_5_EQ.pdResult	Other
	DYH_20240607_5_L.mzML	Mzml
	DYH_20241016_D_5_W.msf	Msf
	DYH_20241016_D_5_W.mzML	Mzml