Membrane mimetic thermal proteome profiling (MM-TPP) towards mapping membrane protein-ligand dynamics

ABSTRACT: We combine the membrane mimetic (MM) Peptidisc with thermal proteome profiling (TPP) to screen membrane proteomes in detergent-free environments. Using whole mouse liver, we demonstrate the stabilization of integral membrane proteins (IMPs), specifically ATP-binding cassette transporters, through interactions with ATP and orthovanadate. Additionally, we assess the downstream effects of ATP hydrolysis products on IMP stability, such as that of GPCR purinergic receptors, and validate novel interactions with AlphaFold3

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Rupinder Jandu  

LAB HEAD: Franck Duong van Hoa

PROVIDER: PXD055093 | Pride | 2025-11-17

REPOSITORIES: Pride