The phosphoproteome of recombinant 2G12 and 353/11 mAb producing CHO-K1 cell lines

ABSTRACT: In this study, we conducted a phosphoproteomic analysis of two cell lines with differing monoclonal antibody (mAb) production levels using label-free quantification mass spectrometry (LFQ-MS). The high-producing cell line, 353/11, was compared to the lower-producing 2G12 cell line. Phosphoproteomic samples were collected on day six of cell culture. To ensure synchronized sampling, all samples were harvested four hours after medium exchange. Additionally, 353/11 cells were separately cultivated and treated in parallel with 1 µg/ml tunicamycin (TM) for four hours to induce mild endoplasmic reticulum (ER) stress.

INSTRUMENT(S):

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Eldi Sulaj  

LAB HEAD: Gorji Marzban

PROVIDER: PXD055200 | Pride | 2025-05-07

REPOSITORIES: Pride