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The phosphoproteome of recombinant 2G12 and 353/11 mAb producing CHO-K1 cell lines

ABSTRACT: In this study, we conducted a phosphoproteomic analysis of two cell lines with differing monoclonal antibody (mAb) production levels using label-free quantification mass spectrometry (LFQ-MS). The high-producing cell line, 353/11, was compared to the lower-producing 2G12 cell line. Phosphoproteomic samples were collected on day six of cell culture. To ensure synchronized sampling, all samples were harvested four hours after medium exchange. Additionally, 353/11 cells were separately cultivated and treated in parallel with 1 µg/ml tunicamycin (TM) for four hours to induce mild endoplasmic reticulum (ER) stress.

INSTRUMENT(S):

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Eldi Sulaj

LAB HEAD: Gorji Marzban

PROVIDER: PXD055200 | Pride | 2025-05-07

REPOSITORIES: Pride

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Publications

Systems Biology of Recombinant 2G12 and 353/11 mAb Production in CHO-K1 Cell Lines at Phosphoproteome Level.

Sulaj Eldi E Sandell Felix L FL Schwaigerlehner Linda L Marzban Gorji G Dohm Juliane C JC Kunert Renate R

Proteomes 20250210 1

<b>Background</b>: Chinese hamster ovary (CHO) cells are extensively used in the pharmaceutical industry for producing complex proteins, primarily because of their ability to perform human-like post-translational modifications. However, the efficiency of high-quality protein production can vary significantly for monoclonal antibody-producing cell lines, within the CHO host cell lines or by extrinsic factors. <b>Methods</b>: To investigate the complex cellular mechanisms underlying this variabili ...[more]

PMID: 39982319

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