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Multi tissue proteomic analysis of cold exposure

ABSTRACT: This study explored the proteomic basis of cold adaptation, quantifying 11,394 proteins across 6 metabolic tissues and plasma. Systems level analysis revealed an enrichment for proteins involved in metabolism. In brown adipose tissue (BAT), cold adaptation was associated with enhanced glucose utilisation, including select remodelling of upper glycolysis to enhance superoxide production and UCP1 activity, and a downregulation of proteins involved in fatty acid oxidation. Strikingly, opposite changes were observed in liver and white adipose tissues, including upregulation of proteins involved in gluconeogenesis and lipolysis, respectively. This suggests that coordinated changes in many organs are required for maximal BAT thermogenesis. This coordination also associated with cold-regulated alterations to the plasma proteome, including elevated Fetuin A that is consistent with enhanced lipolysis in white adipose. Finally, overlapping but inverse changes in cold-exposed and diet-induced obese mice support impaired BAT activity as a driver of obesity.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Skeletal Muscle, Blood Plasma, Brown Adipose Tissue, Liver, White Adipose Tissue, Hypothalamus

SUBMITTER: David James

LAB HEAD: David Ernest James

PROVIDER: PXD055965 | Pride | 2025-04-22

REPOSITORIES: Pride

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			Action	DRS
	20200117_QEHFX3_SJ_DIA_Hypoth_1.8.1_DIA-NN_pg.matrix.tsv	Tabular
	20200117_QEHFX3_SJ_DIA_Hypoth_TN_ID1.raw	Raw
	20200117_QEHFX3_SJ_DIA_Hypoth_TN_ID2.raw	Raw
	20200117_QEHFX3_SJ_DIA_Hypoth_TN_ID3.raw	Raw
	20200117_QEHFX3_SJ_DIA_Hypoth_TN_ID4.raw	Raw

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