Dataset Information

Human whole blood from patients with NSCLC and matching controls processed using VAMS device.

ABSTRACT: Frozen whole blood cell pellets from NSCLC patients and healthy controls were processed using novel techniques to produce a unique sample using VAMS devices. Samples were analysed using liquid chromatography mass spectrometry to collectively screen 3914 proteins from the whole blood sample. Analysis of all fractions produced a set of 515 differentially expressed proteins. Boosted regression tree analysis of the differentially expressed proteins produced a panel of 13 proteins that were able to discriminate between controls and NSCLC patients with an area under the ROC curve (AUC) of 0.864 for the set. Our rapid and reproducible blood preparation and analysis methods enabled the production of high-quality data from small volumes of complex samples that are typically require significant fractionation prior to proteomic analysis.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood

DISEASE(S): Non-small Cell Lung Carcinoma

SUBMITTER: Elisabeth Karsten

LAB HEAD: Ben Herbert

PROVIDER: PXD056469 | Pride | 2025-06-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20220921_QEHFX3_NL_B1_01_T1.raw	Raw
	20220921_QEHFX3_NL_B1_02_T1.raw	Raw
	20220921_QEHFX3_NL_B1_03_T1.raw	Raw
	20220921_QEHFX3_NL_B1_04_T1.raw	Raw
	20220921_QEHFX3_NL_B1_05_T1.raw	Raw

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Publications

Investigating the Use of Novel Blood Processing Methods to Boost the Identification of Biomarkers for Non-Small Cell Lung Cancer: A Proof of Concept.

McMahon Rosalee R Lucas Natasha N Hill Cameron C Pascovici Dana D Herbert Ben B Karsten Elisabeth E

Journal of proteome research 20241206 1

Diagnosis of non-small cell lung cancer (NSCLC) currently relies on imaging; however, these methods are not effective for detecting early stage disease. Investigating blood-based protein biomarkers aims to simplify the diagnostic process and identify disease-associated changes before they can be seen by using imaging techniques. In this study, plasma and frozen whole blood cell pellets from NSCLC patients and healthy controls were processed using both classical and novel techniques to produce a ...[more]

PMID: 39642266

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