Proteomics

Dataset Information

0

Mass spectrometric analysis of the AIBP TurboID proteins


ABSTRACT: AIBP-TurboID-HA expressed HUVECs were seaded into 6-well plates. Once they approximately 80% confluence, cells were incubated with biotin-labeling buffer containing 500 umol/L biotin and 200 umol/L ATP. Cell lysate were collected at 6h with or without biotin incubation. The above cell lysate was incubated with streptavidin magnetic beads overnight at 4℃, Beads were collected for the following mass spectrometry analysis after washing beads for twice.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Lingping Zhu  

LAB HEAD: lingping Zhu

PROVIDER: PXD056677 | Pride | 2025-10-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AIBP_with_boitin-01.raw Raw
AIBP_without_boitin-02.raw Raw
checksum.txt Txt
search.zip Other
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