Mass spectrometric analysis of the AIBP TurboID proteins
Ontology highlight
ABSTRACT: AIBP-TurboID-HA expressed HUVECs were seaded into 6-well plates. Once they approximately 80% confluence, cells were incubated with biotin-labeling buffer containing 500 umol/L biotin and 200 umol/L ATP. Cell lysate were collected at 6h with or without biotin incubation. The above cell lysate was incubated with streptavidin magnetic beads overnight at 4℃, Beads were collected for the following mass spectrometry analysis after washing beads for twice.
INSTRUMENT(S):
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER:
Lingping Zhu
LAB HEAD: lingping Zhu
PROVIDER: PXD056677 | Pride | 2025-10-07
REPOSITORIES: Pride
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