Comprehensive Analysis of leaf cuticle proteome from B. juncea, B. nigra, B. rapa, and R. sativus leaf shed lights on diverse functions of differentially abundant classical and non-classical secretory targets Comprehensive Analysis of leaf cuticle proteome from B. juncea, B. nigra, B. rapa, and R. sativus leaf shed lights on diverse functions of differentially abundant classical and non-classical secretory targets

ABSTRACT: The primary sensor for environmental cues, the cuticle is an essential plant agronomical trait in crop plants. The extracellular cuticle proteome in crop plants is little understood. We performed a quantitative shotgun proteomics analysis on four economically relevant Brassicaceae members: B. juncea, B. nigra, B. rapa, and R. sativus. SignalP and SecretomeP predicted 323 secretory cuticle proteins, of which, 183 proteins were classically secreted and 140 proteins were non-classically secreted. QuickGO analysis revealed that most of these proteins had metabolic activity related to proteins (14%) and lipids (14%) as well as catalytic activity (19%) and defence response roles (18%). Some of the most abundant secreted proteins linked with lipid metabolism (GDSL esterase lipase, ESM1), catalytic activity (glucanase, myrosinase), and oxidoreductases (peroxidase, ascorbate peroxidase) were validated by biochemical investigation and western blotting. Also, the abundant non-classical secreted proteins including malate dehydrogenase, bisphosphatase, catalase, and fructose 1-6 showed in vitro activities. According to the results of the current proteomics study, the active enzymes in the cuticle may serve a variety of functions at the first line of defence. Cutinsomes (esterified nanostructures) which are thought to be involved in cuticle assembly through non enzymatic mechanism were synthesized invitro and validated by FTIR and UV visible spectroscopy. We proposed that cuticle proteins and cutinsomes co-ordinately might be involved in cuticle depositions on the Brassica leaves. In future, cutinsomes may thus also be studied for exploring their applications such as Bioplastics from underutilized Bioresources in India like Brassica agrowastes to reduce environmental pollution.

INSTRUMENT(S):

ORGANISM(S): Brassica Rapa Raphanus Sativus Brassica Juncea Brassica Nigra

TISSUE(S): Plant Cell, True Leaf

SUBMITTER: Renu Deswal  

LAB HEAD: Prof. Renu Deswal

PROVIDER: PXD056855 | Pride | 2025-07-30

REPOSITORIES: Pride