Proteomics

Dataset Information

0

Identification of mitochondrial Cyclophilins

ABSTRACT: We aimed at identify variants of the mitochondrial Cyclophilin D from total lysates of human skin fibroblasts and murine tissues. Total lysates were loaded onto SDS-PAGE stained with MS-compatible Coomassie and bands around 19 and 18 kDa were manually excised. In fact in Western blots analysis of CyPD, which is the sole known mitochondrial Cyclophilin, often show two distinct bands at that molecular weights.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Heart, Skin

SUBMITTER: Gabriele Coluccino  

LAB HEAD: Alessandra Corazza

PROVIDER: PXD057203 | Pride | 2025-05-07

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20230310_Sample_S1.raw Raw
20230310_Sample_S2.raw Raw
20230425_Gel1_Sample_5.raw Raw
20230425_Gel1_Sample_6.raw Raw
20230427_Gel2_Sample_13.raw Raw
Items per page:
1 - 5 of 7
altmetric image

Publications

Cyclophilin (CyP) D is a regulator of the mitochondrial F-ATP synthase. Here we report the discovery of a form of CyPD lacking the first 10 (mouse) or 13 (human) N-terminal residues (ΔN-CyPD), a protein region with species-specific features. NMR studies on recombinant human full-length CyPD (FL-CyPD) and ΔN-CyPD form revealed that the N-terminus is highly flexible, in contrast with the rigid globular part. We have studied the interactions of FL and ΔN-CyPD with F-ATP synthase at the OSCP subunit  ...[more]

Similar Datasets

Proteomics Immunoreactive proteins in the oesophageal gland cells of Anisakis simplex sensu stricto detected by MALDI-TOF/TOF analysis
2021-09-09 | PXD019580 | Pride
Transcriptomics Disrupted iron homeostasis causes dopaminergic neurodegeneration in mice
2015-03-11 | E-GEOD-66730 | biostudies-arrayexpress
Transcriptomics Regulation of microRNA-mediated developmental changes by the SWR1 chromatin remodeling complex in Arabidopsis thaliana
2016-05-31 | E-MTAB-4498 | biostudies-arrayexpress
Proteomics Identification of a novel Glis3-348aa protein in MIN6 cells by LC-MS
2023-11-02 | PXD037447 | Pride
Genomics RNA sequencing of total RNA from spinal cord total lysates of the PS19 tauopathy mouse model.
| PRJNA773047 | ENA
Genomics RNA sequencing from hippocampal total lysates of the rTg4510 tauopathy mouse model.
| PRJNA772726 | ENA
Transcriptomics Next Generation Sequencing of RNAs immunoprecipitated with Cyt c under hyperosmotic stress
2014-05-09 | GSE57435 | GEO
Genomics Mus musculus
| PRJNA314843 | ENA
Genomics C57Bl6J retina lysates
| PRJNA870946 | ENA
Proteomics Genetic analysis of MA4079 - Genetic analysis of MA4079, an aldehyde dehydrogenase homolog, in Methanosarcina acetivorans
2011-07-13 | PRD000398 | Pride