Dataset Information

Proteomics to reveal relationship between menstrual cycle and subjective conditions: a pilot study

ABSTRACT: The menstrual cycle is a vital aspect of women’s health, influencing both physical and mental well-being. Many women experience premenstrual symptom (PMS), such as stomachache and irritability, which can impact daily life. However, the underlying biological mechanisms remain poorly understood, indicating a need for further research. This study aimed to explore the relationship between proteins and subjective condition related to menstrual cycle using two-dimensional gel electrophoresis (2-DE) technique. Serum samples were collected weekly from 42 female students over a period of 5 weeks, resulting in a total of 210 2-DE images. At the same time, two female hormones -- estradiol and progesterone -- were measured. On the morning of every blood collection day, participants rated their subjective condition (SC) score on a scale of 1-5, with scores closer to 5 indicating better condition. These data were categorized into three groups: scores of 1-2 as SC Low, a score of 3 as SC Mid, and scores of 4-5 as SC High. The Steel-Dwass test comparing these groups revealed that two proteins related to oxidative stress, kallistatin and transferrin, are significantly higher in the SC high group compared to both the SC Low and SC Mid groups. Although the specific symptoms that primarily affect SC are not known, oxidative stress is believed to be associated with fatigue and migraine. This study demonstrates that proteins linked to oxidative stress reflect women’s subjective condition during the menstrual cycle. Notably, the evaluation of discernment ability using receiver operating characteristic curve (ROC) shows kallistatin and transferrin exhibit greater discernment than conventional hormonal markers for identifying negative or positive conditions. Investigating well-being related to the menstrual cycle through the lens of proteomics proves to be valuable approach, and targeting these proteins may lead to effective health management strategies for women.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Kai Fushimi

LAB HEAD: Kai Fushimi

PROVIDER: PXD057655 | Pride | 2026-06-15

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 7

Publications

Proteomics to reveal relationship between menstrual cycle and subjective conditions.

Fushimi Kai K Kondo Tasuku T Ikegami Nodoka N Yamada Mizuki M Wong Sing Ying SY Sakamaki-Sunaga Mikako M Hayashi Nobuhiro N

Scientific reports 20260415 1

PMID: 41986428

Similar Datasets

Project description:The Asian citrus psyllid (Diaphorina citri) is a pest of citrus and the primary insect vector of the bacterial pathogen, ‘Candidatus Liberibacter asiaticus’ (CLas), which is associated with citrus greening disease. Variability in CLas titer in insects collected from infected plants has been attributed in part to the host plant from which the insects were collected. CLas accumulates to high titers in infected Citrus macrophylla, and in D. citri feeding on the infected plants of this species. In contrast, in the citrus relative Murraya paniculata, CLas titers remain low in infected plants and in D. citri exposed to infected plants. In this study, top-down and bottom-up proteomics methods were used to investigate the impact of these different host plants on D. citri protein expression. Difference in gel electrophoresis (DIGE) was used to identify protein spots on two-dimensional gels that were larger in one of three insect sample classes compared to the other two: D. citri continuously reared on C. macrophylla, D. citri reared continuously on M. paniculata, and D. citri transferred to M. paniculata for five days feeding after continuous rearing on C. macrophylla. Peptide mass spectrometry was used to identify and quantify proteins in target spots upregulated in each sample class. Shotgun proteomics was used to identify and quantify proteins from analysis of tryptic peptide samples prepared from whole insects from four sample classes: the reciprocal host switch condition (D. citri transferred to C. macrophylla for five days feeding after continuous rearing on M. paniculata) in addition to the three sample classes used in DIGE analysis. Integration of the results of both analyses reveals proteins identified by separate experimental workflows to be upregulated in insects adapted to each host plant, and in insects adapting to a novel host plant. A peptidoglycan-degrading protein involved in the immune response to bacterial pathogens was found to be upregulated in M. paniculata-reared D. citri. In the absence of CLas infection, host plant factors specific to M. paniculata may prime the antibacterial immune response in D. citri. Understanding the insect proteins involved in the adaptation of D. citri to host plants with variation in their susceptibility to CLas will inform the development of control strategies aimed at stopping the spread of citrus greening disease.

			Action	DRS
	251.mzML	Mzml
	251.mzid.gz	Mzid
	251.raw	Raw
	392.mzML	Mzml
	392.mzid.gz	Mzid