Proteomics

Dataset Information

0

Tracking Proteasome Degradation: A Cross-Organ Analysis via Intact degradomics Top-Down Mass Spectrometry

ABSTRACT: Identifying the peptides generated by the proteasome is essential for understanding its role in regulating critical cellular processes such as apoptosis, signaling, and immune responses. In this study, we introduce an enzymatic top-down mass spectrometry approach that allows real-time monitoring of proteasome-mediated cleavage, providing detailed insights into substrate degradation patterns. By examining proteasomes from different organs, we reveal organ-specific peptide production and degradation behaviors, offering a deeper understanding of how proteasome subtypes affect cellular function. This method is well-suited for investigating the influence of proteasome regulators, inhibitors and activators, on proteasome activity. Additionally, its versatility makes it applicable to other proteolytic complexes, paving the way for further research on proteostasis systems.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen, Brain, Liver

SUBMITTER: Katharina Zittlau  

LAB HEAD: Michal Sharon

PROVIDER: PXD058121 | Pride | 2025-08-25

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
F1_F2_SF3_SF4_SF5_SF6_AlphaSyn_20S_60min_R1.RAW Raw
F1_F2_SF3_SF4_SF5_SF6_AlphaSyn_20S_60min_R2.RAW Raw
F1_F2_SF3_SF4_SF5_SF6_AlphaSyn_20S_60min_R3.RAW Raw
F1_MSMS_PrecursorMZ_1095.RAW Raw
F1_MSMS_PrecursorMZ_1228.RAW Raw
Items per page:
1 - 5 of 105
altmetric image

Publications

Tracking proteasome degradation: A cross-organ analysis via intact degradomics mass spectrometry.

Zittlau Katharina I KI   Zachor-Movshovitz Daniel D   Leushkin Yegor Y   Schimmel Brener Roy R   Morgenstern David D   Ben-Nissan Gili G   Sharon Michal M  

Proceedings of the National Academy of Sciences of the United States of America 20250218 8

The proteasome is a multisubunit degradation machinery that is essential for maintaining protein homeostasis by breaking down unnecessary or damaged proteins into peptides. While most of these peptides are further processed into amino acids, a subset evades complete degradation and plays key roles in biological processes such as antigen presentation, signaling, and apoptosis. However, the variability in peptide lengths and the diverse composition of proteasomes make their comprehensive identific  ...[more]

Similar Datasets

Proteomics Identification of Alpha-Synuclein Interacting Synaptosomal Proteins
2015-02-09 | PXD001462 | Pride
Proteomics Charge site manipulation to enhance top-down fragmentation efficiency
2023-06-27 | PXD039993 | Pride
Proteomics Proteome-wide structural changes measured with limited proteolysis-mass spectrometry: an advanced protocol for high-throughput applications.
2023-01-10 | PXD031627 | Pride
Proteomics Beta-synuclein potentiates synaptic vesicle dopamine uptake and rescues dopaminergic neurons from MPTP-induced death in the absence of other synucleins
2022-02-17 | PXD027786 | Pride
Proteomics Proteome-wide structural changes measured with 2 limited proteolysis-mass spectrometry: an advanced 3 protocol for high-throughput applications.
2023-01-10 | PXD031616 | Pride
Transcriptomics Transcription profiling of transgenic mice overexpressing human alpha-synuclein under the PDGF beta promoter to wildtype littermates at 3 months and 9 months of age.
2007-12-20 | E-MEXP-1383 | biostudies-arrayexpress
Transcriptomics Expression data from oligodendroglial cell line
2013-10-28 | E-GEOD-51781 | biostudies-arrayexpress
Proteomics Chemical Crosslinking of Isotopically Labeled α-Synuclein Reveals Structural Changes and the Interface of Oligomeric Species
2023-04-07 | PXD027349 | Pride
Proteomics In Situ Proximity Labeling Identifies Lewy Pathology Molecular Interactions in the Human Brain
2022-02-22 | PXD028830 | Pride
Transcriptomics Bortezomib resistance in CCRF-CEM cell line [CGH]
2015-11-04 | E-GEOD-74633 | biostudies-arrayexpress