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LiP-MS on CHEK1 Purified from HEK293 cells treated with Rabusertib

ABSTRACT: Limited proteolysis coupled to mass spectrometry (LiP-MS) is a structural proteomics technique that can be used to identify the targets of small molecules. This is achieved by incubating lysate with the drug of interest, which induces structural changes such as occupation of the binding site or changes and protein-protein interactions. This is followed by a limited proteolysis step in which the unspecific protease proteinase K is added to the treated lysate. The protease preferentially cleaves accessible and flexible regions of the proteins, thus generating peptides that reflect the changes in surface accessibility caused by the treatment with the small molecule. Here we treated CHEK1 Purified from HEK293 cells with the probe Rabusertib and staurosporine to profile structural effects. This project is part of the IMI EUbOPEN project.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Early Embryonic Cell

SUBMITTER: Viviane Reber

LAB HEAD: Matthias Gstaiger

PROVIDER: PXD058318 | Pride | 2025-04-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	200320_human_PK_iRT.fasta	Fasta
	20240501_200923_VR_Ex56_dirDIA.sne	Other
	20240502_150049_VR_Ex56_dirDIA_Report.tsv	Tabular
	240429_LU1_reberv_Ex56_1.raw	Raw
	240429_LU1_reberv_Ex56_2.raw	Raw

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