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LiP-MS on CAMKK2 Purified from HEK293 cells treated with SGC-CAMKK2-1

ABSTRACT: Limited proteolysis coupled to mass spectrometry (LiP-MS) is a structural proteomics technique that can be used to identify the targets of small molecules. This is achieved by incubating lysate with the drug of interest, which induces structural changes such as occupation of the binding site or changes and protein-protein interactions. This is followed by a limited proteolysis step in which the unspecific protease proteinase K is added to the treated lysate. The protease preferentially cleaves accessible and flexible regions of the proteins, thus generating peptides that reflect the changes in surface accessibility caused by the treatment with the small molecule. Here we treated CAMKK2 Purified from HEK293 cells with the probe SGC-CAMKK2-1, negative control SGC-CAMKK2-1N and staurosporine to profile structural effects. This project is part of the IMI EUbOPEN project.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Early Embryonic Cell

SUBMITTER: Viviane Reber

LAB HEAD: Matthias Gstaiger

PROVIDER: PXD058369 | Pride | 2025-04-30

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	200320_human_PK_iRT.fasta	Fasta
	20230418_171529_VR_Ex33_LiP.sne	Other
	20230418_171645_VR_Ex33_LiP_Report.xls	Xls
	20230419_075504_VR_Ex33_TC.sne	Other
	20230419_080449_VR_Ex33_TC_Report.xls	Xls

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