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Diurnal glycogen metabolism drives rhythmic liver protein secretion

ABSTRACT: The liver secretes most circulating proteins, which are critical for inter-organ communication and systemic energy homeostasis. However, the regulatory mechanisms governing hepatic protein secretion are largely unknown. Using proteomics approaches in humans and mice, we here demonstrate that hepatic protein secretion follows a 24-hour rhythm which is modulated by the timing of food intake. Rhythmic variations in the expression of secretory pathway proteins, involved in protein glycosylation and folding in the endoplasmic reticulum and Golgi apparatus, mediate this process. We find that feeding rhythms and the circadian clock protein BMAL1 regulate diurnal hepatic protein secretion by driving glycogen breakdown which generates glycosylation substrates. Rhythmic secretion was attenuated in a mouse model of obesity and affected by genetic variants associated with glycogen storage disease and congenital disorders of glycosylation. Our study reveals a mechanistic link between glycogen-derived metabolites and protein secretion, thereby connecting nutrient intake with fundamental liver functions.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood Plasma

SUBMITTER: Mark Larance

LAB HEAD: Mark Larance

PROVIDER: PXD058564 | Pride | 2026-01-06

REPOSITORIES: Pride

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			Action	DRS
	081121_BW_EXP2_2_BLANK1.wiff	Wiff
	081121_BW_EXP2_2_BLANK1.wiff.scan	Wiff
	081121_BW_EXP2_2_KD_NRF_ZT0.wiff	Wiff
	081121_BW_EXP2_2_KD_NRF_ZT0.wiff.scan	Wiff
	081121_BW_EXP2_2_KD_NRF_ZT10.wiff	Wiff

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