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Analysis of proteins proximal to epsin 1 in A549 cells during entry of influenza A virus

ABSTRACT: Influenza A viruses (IAVs) are respiratory pathogens that represent a global concern due to their ability to cause seasonal epidemics and sporadic pandemics in the human population. Thus, a comprehensive understanding of IAV biology is essential in order to design effective antivirals. It is well established that entry of most IAVs begins through binding to cell surface sialic acid, however the identity of receptors that mediate virus internalization remains unclear. Here, we performed TurboID-based proximity labelling centered on epsin 1, a protein required for clathrin-mediated endocytosis of IAV, to uncover internalization receptors. By performing affinity-based purification of biotinylated proteins coupled to mass spectrometry, we identified 34 proteins proximal to epsin 1 during IAV infection. Therein we found 11 high-confidence STRING interactors for epsin 1 and 3 putative pro-viral host factors for IAV infection, validating our approach.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Influenza A Virus (a/wsn/1933(h1n1))

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Influenza

SUBMITTER: Milagros Sempere Borau

LAB HEAD: Silke Stertz

PROVIDER: PXD058908 | Pride | 2025-05-08

REPOSITORIES: pride

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			Action	DRS
	2021-09-27-decoys-reviewed-contam-UP000005640-UP000000834.fas	Other
	20210924_C26186_002_S317328_MSET-_Control.raw	Raw
	20210924_C26186_004_S317330_MSGT-_Control.raw	Raw
	20210924_C26186_006_S317329_MSETplus_Treated.raw	Raw
	20210924_C26186_008_S317331_MSGTplus_Treated.raw	Raw

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